Lineage Tracing Using Cux2-Cre and Cux2-CreERT2 Mice
Lineage Tracing Using Cux2-Cre and Cux2-CreERT2 Mice
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DOI：
10.1016/j.neuron.2015.04.019
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发表时间：
2015-05-20
期刊：
影响因子：
16.2
通讯作者：
Mueller, Ulrich
中科院分区：
文献类型：
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作者：
Gil-Sanz, Cristina;Espinosa, Ana;Mueller, Ulrich
通过对Cux2-Cre和Cux2-CreERT2小鼠的遗传命运定位，我们证明了新皮质脑室带(VZ)包含具有受限命运潜力的放射状胶质细胞(RGC)(Franco等人，2012)。使用相同的小鼠品系，郭等人。(2013)得出结论，新皮质VZ不包含血统受限的RGC。我们现在表明，Cux2-Cre/CreERT2小鼠中的重组模式取决于遗传背景和育种策略。我们提供的证据表明，郭美美等人。可能得出了不同的结论，因为他们研究的转基因亚系具有漂移的转基因表达模式。在概括了内源性Cux2表达模式的Cux2-Cre和Cux2-CreERT2小鼠中，绝大多数命运映射神经元表达Satb2而不表达Ctip2，证实了所有新皮质投射神经元的一个受限子集属于Cux2谱系。这篇有关事项的论文是对郭等人的回应。(2013)，发表在《神经元》杂志上。另见埃克勒等人的《引起的事项》答复文件。(2015)，与此同时发表的《神经元上的问题》。
Using genetic fate-mapping with Cux2-Cre and Cux2-CreERT2 mice we demonstrated that the neocortical ventricular zone (VZ) contains radial glial cells (RGCs) with restricted fate potentials (Franco et al., 2012). Using the same mouse lines, Guo et al. (2013) concluded that the neocortical VZ does not contain lineage-restricted RGCs. We now show that the recombination pattern in Cux2-Cre/CreERT2 mice depends on genetic background and breeding strategies. We provide evidence that Guo et al. likely reached different conclusions because they worked with transgenic sublines with drifted transgene expression patterns. In Cux2-Cre and Cux2-CreERT2 mice that recapitulate the endogenous Cux2 expression-pattern, the vast majority of fate-mapped neurons express Satb2 but not Ctip2, confirming that a restricted subset of all neocortical projection neurons belongs to the Cux2 lineage. This Matters Arising paper is in response to Guo et al. (2013), published in Neuron. See also the Matters Arising Response paper by Eckler et al. (2015), published concurrently with this Matters Arising in Neuron.