Role of phosphorylation on characteristics of glycogen phosphorylase in lamb with different glycolytic rates post-mortem
Role of phosphorylation on characteristics of glycogen phosphorylase in lamb with different glycolytic rates post-mortem
复制标题
磷酸化对不同糖酵解率羔羊宰后糖原磷酸化酶特性的影响
DOI：
10.1016/j.meatsci.2020.108096
复制
发表时间：
2020
期刊：
影响因子：
7.1
通讯作者：
Ijaz Muawuz
中科院分区：
文献类型：
--
作者：
Bai Yuqiang;Li Xin;Zhang Dequan;Chen Li;Hou Chengli;Zheng Xiaochun;Ren Chi;Ijaz Muawuz
研究了死后胸长肌糖原磷酸化酶活性与磷酸化水平的关系。在宰后0.5 h、2 h、6 h、12 h、24 h、48 h和72 h采集60只羔羊样品，根据宰后6 h pH值将样品分为3组（n= 6），每组6只，分别采用不同的糖酵解速率（快、中、慢）。测定磷酸化水平、糖原磷酸化酶活性和表达。结果表明，在死后24h内，pH缓慢下降组的糖原磷酸化水平和糖原磷酸化酶活性均低于pH快速下降组（P< .05）。糖原磷酸化酶活性与磷酸化水平呈显著正相关。总之，这些数据表明，糖原磷酸化酶活性在羔羊的磷酸化水平和死后时间的影响。
The relationship between glycogen phosphorylase activity and phosphorylation levels in thelongissimusthoracismuscle post-mortem was studied. Sixty lamb samples were collected at 0.5 h, 2 h, 6 h, 12 h, 24 h, 48 h, and 72 h post-mortem and divided into three groups (n= 6) with different glycolytic rates (fast, intermediate, and slow) according to the pH at 6 h post-mortem. The phosphorylation level and activity and expression of glycogen phosphorylase were determined. The results showed that the phosphorylation level and activity of glycogen phosphorylase in the slow pH decline group was lower than that in the fast pH decline group during 24 h post-mortem (P< .05). There was a significant positive correlation between the glycogen phosphorylase activity and the phosphorylation level. In conclusion, these data demonstrated that the glycogen phosphorylase activity in lambs was affected by phosphorylation levels and postmortem duration.