MORPHOLOGICAL ANALYSIS OF HONEYBEE ANTENNAL CELLS GROWING IN PRIMARY CULTURES
MORPHOLOGICAL ANALYSIS OF HONEYBEE ANTENNAL CELLS GROWING IN PRIMARY CULTURES
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DOI：
10.1016/0040-8166(94)90007-8
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发表时间：
1994-08-01
期刊：
影响因子：
2.6
通讯作者：
BEADLE, DJ
中科院分区：
文献类型：
--
作者：
GASCUEL, J;MASSON, C;BEADLE, DJ
本文报道了蜜蜂触角细胞的体外培养技术。根据形态学和免疫细胞化学标准，可将培养的细胞分为神经细胞和非神经细胞。神经细胞（D型）具有昆虫嗅觉受体神经元（ORN）的主要形态特征。非神经细胞是大而扁平的细胞，可分为三种主要类型：A、B和C型细胞。A型细胞为梭形细胞，类似于培养的昆虫肌细胞。B型细胞为大细胞，胞质呈面纱状。这些细胞倾向于聚集并向细胞聚集体的中心空泡化。C型细胞为双极（C1型）或多极（C2型）扁平细胞，与培养物中的昆虫胶质细胞非常相似。
A culture technique for the in vitro growth of antennal cells from honeybee is described. On the basis of morphological and immunocytochemical criteria, the cultured cells could be classified into neural and non-neural cells. Neural cells (type D) exhibited the main morphological features of insect olfactory receptor neurones (ORNs). Non-neural cells were large, flat cells that could be divided into three main types: Type A, B and C cells. Type A cells were spindle-like cells and resembled insect myocytes in culture. Type B cells were large cells with a veil-like cytoplasm. These cells tended to group and vacuolate towards the center of the cellular aggregate. Type C cells were either bipolar (Type C1) or multipolar (Type C2) flat cells which closely resembled insect glial cells in cultures.