Coordinate regulation of bacterial virulence genes by a novel adenylate cyclase-dependent signaling pathway
Coordinate regulation of bacterial virulence genes by a novel adenylate cyclase-dependent signaling pathway
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DOI：
10.1016/s1534-5807(03)00019-4
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发表时间：
2003-02-01
影响因子：
11.8
通讯作者：
Lory, S
中科院分区：
文献类型：
--
作者：
Wolfgang, MC;Lee, VT;Lory, S
III型分泌系统（TTSS）被许多细菌病原体用于将效应蛋白直接注射到宿主细胞中。使用全基因组芯片，我们调查的条件和调控因素，控制铜绿假单胞菌TTSS的表达。已知的TTSS基因的转录反应表明表达的层次模式，其中一组分泌装置和调节基因组成型表达。进一步分析与编码TTSS的基因协调调节的基因，导致鉴定出源自膜相关腺苷酸环化酶并控制TTSS基因表达的信号通路。缺乏合成cAMP或cAMP结合蛋白Vfr的能力的突变体的转录组分析表明，这一途径参与了宿主定向毒力决定因子（包括TTSS）的全局调节。
Type III secretion systems (TTSSs) are utilized by numerous bacterial pathogens to inject effector proteins directly into host cells. Using a whole-genome microarray, we investigated the conditions and regulatory factors that control the expression of the Pseudomonas aeruginosa TTSS. The transcriptional response of known TTSS genes indicates a hierarchical pattern of expression in which a set of secretion apparatus and regulatory genes is constitutively expressed. Further analysis of genes coordinately regulated with those encoding the TTSS led to the identification of a signaling pathway that originates from a membrane-associated adenylate cyclase and controls TTSS gene expression. Transcriptome analysis of mutants lacking the ability to synthesize cAMP or the cAMP binding protein Vfr implicated this pathway in the global regulation of host-directed virulence determinants, including the TTSS.