HLA-F and MHC-I Open Conformers Bind Natural Killer Cell Ig-Like Receptor KIR3DS1.
HLA-F and MHC-I Open Conformers Bind Natural Killer Cell Ig-Like Receptor KIR3DS1.
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DOI：
10.1371/journal.pone.0163297
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Geraghty DE
中科院分区：
文献类型：
--
作者：
Burian A;Wang KL;Finton KA;Lee N;Ishitani A;Strong RK;Geraghty DE
基于先前支持HLA-F作为KIR 3DL 2和KIR 2DS 4配体的发现，我们通过表面等离子体共振测量的相互作用研究了MHC-I开放构象体（OC）作为KIR 3DS 1和KIR 3DL 1配体的潜力。这些测量显示KIR 3DS 1而不是KIR 3DL 1与HLA-F和其他MHC-I OC的物理结合，同时还证实了KIR 3DL 1与MHC-I肽复合物的同种异型特异性结合。从细胞系和生化异源二聚化实验与重组蛋白的生化下拉得到一致的结果。此外，HLA-F和KIR 3DS 1与天然和活化的NK和T细胞的表面结合与推定的配体或受体的特异性表达一致。KIR 3DS 1的功能反应由与结合至表面的HLA-F孵育的活化细胞中颗粒胞吐增加指示。这些数据扩展了MHC-I开放型构象异构体与炎症反应期间表达的活化KIR受体之间相互作用的模型，可能有助于先天性和适应性免疫反应之间的沟通。
Based on previous findings supporting HLA-F as a ligand for KIR3DL2 and KIR2DS4, we investigated the potential for MHC-I open conformers (OCs) as ligands for KIR3DS1 and KIR3DL1 through interactions measured by surface plasmon resonance. These measurements showed physical binding of KIR3DS1 but not KIR3DL1 with HLA-F and other MHC-I OC while also confirming the allotype specific binding of KIR3DL1 with MHC-I peptide complex. Concordant results were obtained with biochemical pull-down from cell lines and biochemical heterodimerization experiments with recombinant proteins. In addition, surface binding of HLA-F and KIR3DS1 to native and activated NK and T cells was coincident with specific expression of the putative ligand or receptor. A functional response of KIR3DS1 was indicated by increased granule exocytosis in activated cells incubated with HLA-F bound to surfaces. The data extend a model for interaction between MHC-I open conformers and activating KIR receptors expressed during an inflammatory response, potentially contributing to communication between the innate and adaptive immune response.
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影响因子：
30.5
作者：
Garcia-Beltran WF;Hölzemer A;Martrus G;Chung AW;Pacheco Y;Simoneau CR;Rucevic M;Lamothe-Molina PA;Pertel T;Kim TE;Dugan H;Alter G;Dechanet-Merville J;Jost S;Carrington M;Altfeld M
通讯作者：
Altfeld M
影响因子：
29.7
作者：
Blum JS;Wearsch PA;Cresswell P
通讯作者：
Cresswell P
DOI：
10.4049/jimmunol.1300081
发表时间：
2013-10-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Goodridge JP;Burian A;Lee N;Geraghty DE
通讯作者：
Geraghty DE
DOI：
10.1073/pnas.95.9.5199
发表时间：
1998-04-28
影响因子：
11.1
作者：
Lee, N;Llano, M;Geraghty, DE
通讯作者：
Geraghty, DE
DOI：
10.1073/pnas.0605228103
发表时间：
2006-10-31
影响因子：
11.1
作者：
Shiroishi, Mitsunori;Kuroki, Kimiko;Maenaka, Katsumi
通讯作者：
Maenaka, Katsumi