Micro-cantilever bending for elastic modulus measurements of a single trabecula in cancellous bone
Micro-cantilever bending for elastic modulus measurements of a single trabecula in cancellous bone
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微悬臂梁弯曲用于测量松质骨中单个小梁的弹性模量
DOI：
10.1016/j.jbiomech.2016.10.016
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发表时间：
2016
影响因子：
2.4
通讯作者：
Koichi Fukasawa
中科院分区：
文献类型：
--
作者：
Satoshi Yamada;Shigeru Tadano;Koichi Fukasawa
在小骨样本（如单个骨小梁）上进行的力学测试仍然具有挑战性，因为它们的隔离、固定和精确加载都很复杂。因此，我们描述了一种新的实验方法来测量一个单一的小梁使用微悬臂梁弯曲（MCB）测试的弹性性能。该方法不要求标本与松质骨完全分离，试验过程中标本可以很容易地固定。在本研究中，总共使用了10个取自成年牛股骨近端骨骺的骨小梁标本。使用小型测试装置进行测量，该装置包括单轴台、测力传感器、光学显微镜和具有锥形孔的小板，该锥形孔用于在样本边缘施加载荷。将每个样本定位在孔的边缘，并通过移动载物台使其变形。通过光学显微镜观察试样的偏转。假设试样的形状为垂直圆柱体，根据力-挠度关系计算试样的弹性模量。结果表明，单个骨小梁的平均弹性模量为9.1±5.4 GPa，包括文献中的值。因此，MCB测试是一种新的简单的方法进行生物力学分析的一个单一的小梁。
Mechanical tests performed on small bone specimens such a single trabecula remain challenging because their isolation, fixation, and precise loading are complicated. Hence, we describe a novel experimental method to measure the elastic properties of a single trabecula using micro-cantilever bending (MCB) testing. The method does not require specimens to be completely separated from the cancellous bone, and the specimen can be easily fixed during the test. In total, 10 trabecular specimens taken from the proximal epiphysis of an adult bovine femur were used in the present study. Measurements were conducted using a small testing device comprising a 1-axial stage, load cell, optical microscope, and small plate with a taper bore for applying load at the edge of the specimen. Each specimen was positioned at the edge of the bore and was deformed by displacing the stage. The deflection of the specimen was observed by optical microscopy. The elastic modulus of the specimen was calculated on the basis of the force–deflection relationship, assuming that the shape of the specimen was a vertical circular cylinder. As a result, an average elastic modulus of 9.1±5.4 GPa was obtained for a single trabecula, including the values in literature. Thus, the MCB test is a novel simple method for biomechanical analysis of a single trabecula.