Co-occurrence of early gut colonization in neonatal piglets with microbiota in the maternal and surrounding delivery environments
Co-occurrence of early gut colonization in neonatal piglets with microbiota in the maternal and surrounding delivery environments
复制标题
新生仔猪早期肠道定植与母体及周围分娩环境中微生物群的共存
DOI：
10.1016/j.anaerobe.2017.12.002
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发表时间：
2018-02-01
期刊：
影响因子：
2.3
通讯作者：
Zhu, Weiyun
中科院分区：
文献类型：
--
作者：
Chen, Xue;Xu, Jumei;Zhu, Weiyun
肠道微生物群的早期发育在宿主健康中起着重要作用;到目前为止，新生仔猪中第一次定植的主要起源在很大程度上尚不清楚。本研究旨在通过Illumina MiSeq测序16 S核糖体RNA基因，研究哺乳期新生仔猪肠道微生物群的早期发育及其与母体和周围环境中微生物的共存。结果表明，仔猪粪便中微生物的丰富度和多样性从出生到断奶（21 d）显著增加。仔猪出生后粪便中的微生物群落组成和功能与地板（FL）、母猪乳汁（SM）和乳头表面（SN）中的微生物群落组成和功能相似，泌乳后期仔猪粪便中的微生物群落更接近母体。SourceTracker分析表明，来自FL、SM和SN的微生物群最有可能是新生儿胃肠道的最早乘客，但在哺乳期间停留时间不长。母猪粪便菌群通过与前定居者的共现效应更容易在新生仔猪肠道中定居。这项研究表明，来自母体和周围环境的微生物可能在新生仔猪出生后的微生物演替中发挥重要作用。(C)2017爱思唯尔有限公司版权所有
The early development of gut microbiota plays a fundamental role in host health; so far, the main origins of the first colonization in newborn piglets are largely unclear. This study aimed to investigate the early development of gut microbiota in newborn piglets during lactation and their co-occurrence with microbes in the maternal and surrounding environments by Illumina MiSeq sequencing of 16S ribosomal RNA genes. The results showed that the microbial richness and diversity in piglets' feces (PF) significantly increased from birth to weaning (21 d). The composition and function of microbiota in the feces of piglets after birth tended to be similar to those from the slatted floor (FL), sow's milk (SM) and nipple surface (SN), and lacter, the fecal microbial communities of piglets later during lactation were more similar to their mother's. SourceTracker analysis showed that the microbiota from the FL, SM and SN were most likely the earliest passengers to the neonatal gastrointestinal tract, but did not have a long stay during lactation. The sow's fecal microbiota were easier to colonize in newborn piglet's guts via the co-occurrence effect with former settlers. This study suggests that microbes from the maternal and surrounding environments may play an important role in the microbial succession of newborn piglets after birth. (C) 2017 Elsevier Ltd. All rights reserved.