Protein antigen in serotype k Streptococcus mutans clinical isolates
Protein antigen in serotype k Streptococcus mutans clinical isolates
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DOI：
10.1177/154405910808701001
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发表时间：
2008-10-01
影响因子：
7.6
通讯作者：
Ooshima, T.
中科院分区：
文献类型：
--
作者：
Nakano, K.;Nomura, R.;Ooshima, T.
变形链球菌是龋病和感染性心内膜炎的主要病原菌，分为c、e、f、k四种血清型，其中k型是近年来在感染性心内膜炎患者中发现较多的菌株。因此，我们假设与感染性心内膜炎相关的共同特性存在于这些菌株中。对56株口腔变形链球菌进行了检测，其中包括11株k型菌株。Western blotting分析显示3种糖基转移酶在所有菌株中均有表达，而约190 kDa的细胞表面蛋白(PA)在12株菌株中不表达，其中k型的比例较高(7/12)。此外，k血清型菌株的细胞疏水性和吞噬敏感性较低。这些结果表明，PA的缺乏表达、低细胞疏水性和吞噬敏感性是与血清型k型菌株相关的常见细菌特性，这可能与感染性心内膜炎的毒力有关。
Streptococcus mutans, a major pathogen of dental caries and infective endocarditis, is classified into serotypes c, e, f, and k, with serotype k strains recently reported to be frequently detected in persons with infective endocarditis. Thus, we hypothesized that common properties associated with infective endocarditis are present in those strains. Fifty-six oral S. mutans strains, including 11 serotype k strains, were analyzed. Western blotting analysis revealed expression of the 3 types of glucosyltransferases in all strains, while expression of the approximately 190-kDa cell-surface protein (PA) was absent in 12 strains, among which the prevalence of serotype k (7/12) was significantly high. Furthermore, cellular hydrophobicity and phagocytosis susceptibility were lower in the group of serotype k strains. These results indicate that the absence of PA expression, low cellular hydrophobicity, and phagocytosis susceptibility are common bacterial properties associated with serotype k strains, which may be associated with virulence for infective endocarditis.