The effect of endotoxin on in vivo rat alveolar macrophage phagocytosis
The effect of endotoxin on in vivo rat alveolar macrophage phagocytosis
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DOI：
10.3109/01902149809099593
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发表时间：
1998-11-01
影响因子：
1.7
通讯作者：
Brain, JD
中科院分区：
文献类型：
--
作者：
Frevert, CW;Warner, AE;Brain, JD
本研究旨在探讨内毒素血症时肺泡巨噬细胞（AM）吞噬功能是否受损。因此，我们的特点是在体内AM吞噬功能的大鼠静脉内（IV）或intrichelheal（IT）管理脂多糖（LPS）。大鼠静脉注射0、1、2和5 mg/kg LPS后，AM对Au-198胶体的吞噬能力明显降低（P <0.01），支气管肺泡灌洗（BAL）中AM的回收率降低（P = 0.017），灌洗中性粒细胞回收率（P = 0.15）或BAL中白蛋白浓度（P = 0.14）无显著差异。在IT施用的LPS的剂量中（即，0、1、5和10 mg/kg），AM吞噬作用无差异（P = .29），AM恢复率显著降低（P = .002），中性粒细胞数量显著增加（P = .01），对白蛋白浓度影响不大（P = .06）。因此，我们发现内毒素对小鼠的给药不会损害体内AM吞噬功能。事实上，我们的研究结果表明，静脉注射LPS可增加AM对Au-198的吞噬作用。
This study was performed to explore whether alveolar macrophage (AM) phagocytosis would be impaired during endotoxemia. Therefore, we characterized in vivo AM phagocytic function in rats following either intravenous (IV) or intratracheal (IT) administration of lipopolysaccharide (LPS). The IV administration of LPS to rats at dosages of 0, 1, 2, and 5 mg/kg showed that increasing LPS doses were significantly associated with ino eased AM phagocytosis of Au-198 colloid (P < .01), decreased recovery of AMs in bronchoalveolar lavage (BAL) (P = .017), no significant differences in neutrophil recovery by lavage (P = .15), or in the concentration of albumin in BAL (P = .14). Across the dosages of LPS administered IT (i.e., 0, 1, 5, and 10 mg/kg), there was no difference in AM phagocytosis (P = .29), a significant decrease in AM recovery (P = .002), a significant increase in neutrophil number (P = .01), and little effect on the concentration of albumin (P = .06). Thus, we found that the administration of endotoxin to mis did not impair in vivo AM phagocytic function. In fact, our findings suggest that the IV administration of LPS mar increase AM phagocytosis of Au-198.