ASTROVIRUS INFECTION IN VOLUNTEERS
ASTROVIRUS INFECTION IN VOLUNTEERS
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DOI：
10.1002/jmv.1890030308
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发表时间：
1979-01-01
影响因子：
12.7
通讯作者：
REED, SE
中科院分区：
文献类型：
--
作者：
KURTZ, JB;LEE, TW;REED, SE
EM显示，从患有轻度胃肠炎的儿童的粪便中提取的提取物和滤液含有大量的星状病毒颗粒，并给8名志愿者口服。一名受试者出现腹泻并同时在粪便中排出大量星状病毒，另一名受试者有轻微的体质症状，病毒排出水平较低。其他志愿者（9人）被给予腹泻志愿者的粪便滤液，随后2人出现了星状病毒脱落。伴随病毒脱落的综合征似乎不同于与W剂相关的综合征。星状病毒接种者[13/16]随后血清中同源抗体滴度升高。显然，星状病毒引起的传染性感染对成人的致病性较低。将星状病毒接种人胚胎肾细胞，电镜显示其含有28 nm的病毒样颗粒，利用免疫荧光检测粪便中的病毒并测定星状病毒抗体。
An extract and a filtrate prepared from feces of a child with mild gastroenteritis were shown by EM to contain numberous astrovirus particles and were given to 8 volunteers by mouth. A subject developed diarrheal illness and concurrently shed large amounts of astrovirus in feces, and another had mild constitutional symptoms with a lower level of virus shedding. Other volunteers (9) were given fecal filtrate from the volunteer with diarrhea, and astrovirus shedding subsequently occurred in 2. The syndrome accompanying virus shedding appeared distinct from that associated with the W agent. Astrovirus-inoculated subjects [13/16] subsequently developed a rise in titer of the homologous antibody in serum. Apparently, astrovirus causes a transmissible infection that is of low pathogenicity for adults. Immunofluorescence of human embryo kidney cells inoculated with astrovirus and shown by EM to contain 28 nm virus-like particles was used both to detect virus in feces and to assay astrovirus antibody.