Contribution of PPARγ in Modulation of LPS-Induced Reduction of Milk Lipid Synthesis in Bovine Mammary Epithelial Cells
Contribution of PPARγ in Modulation of LPS-Induced Reduction of Milk Lipid Synthesis in Bovine Mammary Epithelial Cells
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PPAR γ 在调节 LPS 诱导的牛乳腺上皮细胞乳脂合成减少中的作用
DOI：
10.17957/ijab/15.1138
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发表时间：
2019-01-01
影响因子：
--
通讯作者：
Wang, Jianfa
中科院分区：
文献类型：
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作者：
He, Xianjing;Lian, Shuai;Wang, Jianfa
牛奶的脂质含量被认为是牛奶质量的一个指标。在泌乳的奶牛中也观察到脂多糖（LPS）诱导的乳脂降低。然而，LPS如何调节乳脂合成尚不完全清楚。我们的目的是研究牛乳腺上皮细胞（bMEC）中的分子机制。用油酸处理bMEC以诱导乳脂合成。通过酶比色法检测bMEC层中的细胞内甘油三酯以及培养基中分泌的甘油三酯。研究了过氧化物酶体增殖物激活受体γ（PPARγ）的表达和活性，以及脂肪酸合成酶（FASN）、乙酰辅酶A羧化酶 - 1（ACACA）、酰基辅酶A结合蛋白（ACBP）和脂蛋白脂肪酶（LPL）的表达。结果表明，LPS通过下调PPARγ的转录、翻译和核转位水平降低了脂质合成水平。结果还表明，LPS处理后bMEC中PPARγ靶基因FASN、ACACA、ACBP和LPL的转录水平显著下调。这些结果表明PPARγ参与了LPS诱导的bMEC中乳脂合成的减少。我们的数据可能为LPS导致乳脂降低的机制提供新的见解。（C）2019 Friends Science Publishers
Lipid content of milk is recognized as an indicator of milk quality. Lipopolysaccharides (LPS)-induced milk fat depression has also been observed in lactating daily cows. However, it is not completely clear how the LPS regulate the milk lipid synthesis? Our objective was to examine the molecular mechanism in bovine mammary epithelial cells (bMEC). The bMEC were treated with oleic acid to induce milk lipid synthesis. The intracellular triglyceride in bMEC layer and the secreted triglyceride in culture medium were detected by enzymatic colorimetric assay. The expression and activity of the peroxisome proliferator-activated receptor gamma (PPAR gamma), the expression of the FASN (fatty acid synthetase), ACACA (acetyl-CoA carboxylase-1), ACBP (acyl-CoA-binding protein), and LPL (lipoprotein lipase) were investigated. The results showed that LPS decreased the level of lipid synthesis via down-regulating the transcription, translation, and nuclear translocation level of the PPAR gamma. The results also indicated that the transcription level of the PPAR gamma target genes, FASN, ACACA, ACBP, and LPL were significantly down-regulated in bMEC after LPS treatment. These results indicate that PPAR gamma participate in LPS-induced reduction of milk lipid synthesis in bMEC. Our data may provide new insight into the mechanisms of milk fat depression caused by LPS. (C) 2019 Friends Science Publishers