Rescue and replication of the adeno-associated virus 2 genome in mortal and immortal human cells.
Rescue and replication of the adeno-associated virus 2 genome in mortal and immortal human cells.
复制标题
腺相关病毒 2 号基因组在永生和永生人类细胞中的拯救和复制。
DOI：
10.1159/000150078
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发表时间：
1989
期刊：
影响因子：
4.6
通讯作者：
Srivastava,A
中科院分区：
文献类型：
--
作者：
Nahreini,P;Srivastava,A
腺相关病毒(AAV)的有效感染以及从重组质粒中拯救AAV基因组及其随后在已建立的细胞系中的复制依赖于与腺病毒的共同感染。在人的死亡细胞和永生细胞中进行了AAV感染和拯救/复制的比较研究。在复制能力有限的正常二倍体细胞中，与具有无限增殖能力的多倍体细胞相比，AAVDNA复制以及子代病毒粒子的组装和释放显著延迟。从重组质粒中抢救AAV基因组及其随后的复制在人类细胞中也同样延迟。AAV拯救/复制的延迟似乎与腺病毒编码的功能无关。AAV复制所需的宿主细胞成分可能是在正常人二倍体细胞中次优诱导的，这为自然AAV感染提供了一个有用的模型系统。
Productive infection of the adeno-associated virus (AAV) as well as rescue of the AAV genome from a recombinant plasmid and its subsequent replication in established cell lines are dependent upon coinfection with adenovirus. A comparative study of AAV infection and rescue/replication was carried out in mortal and immortal human cells. AAV DNA replication as well as assembly and release of the progeny virions were significantly delayed in normal diploid cells, which have a limited replicative potential, compared with polyploid cells, which have an unlimited proliferative potential. Rescue of the AAV genome from a recombinant plasmid and its subsequent replication were similarly delayed in mortal cells. The delay in AAV rescue/replication appeared to be unrelated to the adenovirus-encoded functions. The putative host-cell component required for AAV replication may be suboptimally induced in normal human diploid cells, which offer a useful model system for a natural AAV infection.