Interaction of Myelin Basic Protein with Myelin-like Lipid Monolayers at Air-Water Interface.
Interaction of Myelin Basic Protein with Myelin-like Lipid Monolayers at Air-Water Interface.
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DOI：
10.1021/acs.langmuir.8b00321
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发表时间：
2018-05
期刊：
影响因子：
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通讯作者：
Katharina Widder;Jennica Träger;Andreas Kerth;G. Harauz;D. Hinderberger
中科院分区：
文献类型：
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作者：
Katharina Widder;Jennica Träger;Andreas Kerth;G. Harauz;D. Hinderberger
髓鞘碱性蛋白（MBP）与少突胶质细胞膜细胞质小叶的相互作用对于中枢神经系统髓鞘的形成和压实至关重要，并且异常改变并涉及多发性硬化症等神经退行性疾病的发病机制。为了更详细地了解这种相互作用，我们在空气-水界面通过单层吸附实验研究了MBP对与中枢神经系统髓磷脂组成相似的脂质单层的吸附。测量不同髓鞘样脂质单层的表面压力和相关的MBP最大插入压力，提供了关于每种单一脂质在髓鞘中的具体作用的信息。根据带负电荷的脂质与不带电荷的脂质的比例和电荷之间的距离，发现吸附过程是由两种抵消作用决定的：(i)蛋白质结合，导致表面压力增加；（ii）由于带正电荷的蛋白质和带负电荷的脂质之间的静电相互作用，导致表面压力降低。尽管静电相互作用导致高插入压力，但相关的脂质冷凝降低了髓磷脂样单层的流动性。
Interaction of myelin basic protein (MBP) and the cytoplasmic leaflets of the oligodendrocyte membrane is essential for the formation and compaction of the myelin sheath of the central nervous system and is altered aberrantly and implicated in the pathogenesis of neurodegenerative diseases like multiple sclerosis. To gain more detailed insights into this interaction, the adsorption of MBP to model lipid monolayers of similar composition to the myelin of the central nervous system was studied at the air-water interface with monolayer adsorption experiments. Measuring the surface pressure and the related maximum insertion pressure of MBP for different myelin-like lipid monolayers provided information about the specific role of each of the single lipids in the myelin. Depending on the ratio of negatively charged lipids to uncharged lipids and the distance between charges, the adsorption process was found to be determined by two counteracting effects: (i) protein incorporation, resulting in an increasing surface pressure and (ii) lipid condensation due to electrostatic interaction between the positively charged protein and negatively charged lipids, resulting in a decreasing surface pressure. Although electrostatic interactions led to high insertion pressures, the associated lipid condensation lowered the fluidity of the myelin-like monolayer.