Geranylgeraniol causes a decrease in levels of calreticulin and tyrosine phosphorylation of a 36-kDa protein prior to the appearance of apoptotic features in HL-60 cells.
Geranylgeraniol causes a decrease in levels of calreticulin and tyrosine phosphorylation of a 36-kDa protein prior to the appearance of apoptotic features in HL-60 cells.
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在 HL-60 细胞中出现凋亡特征之前，香叶基香叶醇会导致 36-kDa 蛋白的钙网蛋白和酪氨酸磷酸化水平降低。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
Kazuyasu Nakaya
中科院分区：
文献类型：
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作者：
S. Nakajo;Mitsuru Okamoto;Yutaka Masuda;Itaru Sakai;Shigemitsu Ohsawa;Kazuyasu Nakaya
最近有研究表明，香叶香叶醇（GGO）对包括HL-60细胞在内的多种肿瘤细胞具有强效的诱导凋亡活性。在本研究中，我们发现，在诱导HL-60细胞凋亡之前，GGO可显著抑制钙结合蛋白钙网蛋白的表达。此外，我们还观察到HL-60细胞中一个36 kda蛋白的酪氨酸磷酸化显著降低，该蛋白是酪氨酸磷酸化的主要蛋白。这些发现表明，钙网蛋白水平的降低和36-kDa蛋白酪氨酸磷酸化的降低可能与GGO诱导HL-60细胞凋亡有关。
It was demonstrated recently that geranylgeraniol (GGO) has potent apoptosis-inducing activity in various lines of tumor cells, including HL-60 cells. In the present study, we found that GGO markedly inhibited the expression of a calcium-binding protein, calreticulin, prior to the induction of apoptosis in HL-60 cells. Furthermore, we also observed a significant decrease in the tyrosine phosphorylation of a 36-kDa protein that is a major tyrosine-phosphorylated protein in HL-60 cells. These findings suggest that decreases in levels of calreticulin and in the tyrosine phosphorylation of the 36-kDa protein might be associated with the induction of apoptosis by GGO in HL-60 cells.