Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: Results of a blind test
Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: Results of a blind test
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DOI：
10.1128/jcm.40.11.4161-4165.2002
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发表时间：
2002-11-01
影响因子：
9.4
通讯作者：
Vuitton, DA
中科院分区：
文献类型：
--
作者：
Ito, A;Xiao, N;Vuitton, DA
泡状棘球蚴病（AE）是北方非热带地区最具潜在致死性的寄生虫病，囊状棘球蚴病（CE）也是该地区的地方病。AE和CE在中国都是高度流行的，棘球蚴病的血清学检测，无论是AE还是CE，以及区分AE和CE都是关键问题。使用亲和纯化的和重组的Em18，对Em18抗原的Western印迹分析（WB）和酶联免疫吸附测定（ELISA）进行评价，使用来自法国诊断的患者的60份人血清进行“盲法”。将结果与法国开发的市售棘球蚴WB免疫球蛋白G（IgG）试剂盒进行比较。Em18 WB和棘球蚴WB IgG对AE的检测结果非常相似。亲和纯化的Em18或重组Em18 WB和棘球蚴WB IgG似乎都适用于AE的鉴定，后者似乎适用于AE和CE，而亲和纯化的Em18 ELISA和新开发的重组Em18 ELISA似乎适用于检测AE，特别是流行病学调查。
Alveolar echinococcosis (AE) is the most potentially lethal parasitic zoonosis of the nontropical areas in the northern hemisphere, where cystic echinococcosis (CE) is also endemic. Both AE and CE are highly endemic in China, and both serologic detection of echinococcosis, either AE or CE, and differentiation of AE from CE are crucial problems. Evaluation of Western blot analysis (WB) and enzyme-linked immunosorbent assay (ELISA) for the Em18 antigen, using affinity-purified and recombinant Em18, was carried out "blindly" using 60 human sera from patients diagnosed in France. The results were compared with those obtained using a commercially available Echinococcus WB immunoglobulin G (IgG) kit developed in France. The Em18 WB and Echinococcus WB IgG showed very similar results for detection of AE. Both affinity-purified Em18 or a recombinant Em 18 WB and Echinococcus WB IgG seem useful for identification of AE, and the latter seems appropriate for both AE and CE, whereas affinity-purified Em18 ELISA and the newly developed recombinant Em18 ELISA appear to be suitable for detection of AE, especially for epidemiological surveys.