Feather HSP70: a novel non-invasive molecular marker for monitoring stress induced by heat exposure in broilers
Feather HSP70: a novel non-invasive molecular marker for monitoring stress induced by heat exposure in broilers
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DOI：
10.3382/ps/pez120
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发表时间：
2019-09-01
期刊：
影响因子：
4.4
通讯作者：
Dridi, Sami
中科院分区：
文献类型：
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作者：
Greene, Elizabeth S.;Rajaei-Sharifabadi, Hossein;Dridi, Sami
家禽的健康状况和因热应激（HS）引起的经济负担对家禽业具有重要意义。有效设计和有效评估任何缓解HS对家禽生产不利影响的策略都需要准确的应激测量。然而，目前监测家禽应激的方法并不理想，因为它们是侵入性的或主观的，因此是可变的。在这里，我们证明了HSP70，一个公认的细胞内应激伴侣，在鸡毛中表达，并对HS有反应，因此可以用作应激标记。在美国阿肯色州夏季（2016年6月21日），从同一只鸟（Cobb500, n = 9）的早晨（谷仓温度24℃）和下午（谷仓温度29.5℃）收集生长羽毛。iButton数据温度记录仪显示，下午，这些鸟在喘气，它们的核心体温明显高于早上。与此同时，血液HSP70 mRNA和羽毛HSP70 mRNA和蛋白的表达在下午显著高于上午。同样，下午十二指肠的HSP70蛋白表达也明显高于上午。总之，这些发现确定了羽毛HSP70是一种新的非侵入性分子特征，反映了细胞内和系统应激，可用于监测鸡和其他鸟类物种在不同挑战下的健康状况。
Poultry well-being and economic burden due to heat stress (HS) are of great importance to the poultry industry. Efficient design and effective evaluation of any strategies to alleviate the adverse effects of HS on poultry production require an accurate measurement of stress. However, current methods for monitoring stress in poultry are less than ideal, as they are invasive or subjective, and therefore variable. Here, we demonstrated that HSP70, the well-established intracellular stress chaperone, is expressed in chicken feather and is responsive to HS, and could therefore be used as a stress marker.Growing feathers were collected from the same bird (Cobb500, n = 9) in the morning (barn temperature 24 degrees C) and afternoon (barn temperature 29.5 degrees C) in the summer (2016 June 21) in Arkansas, USA. In the afternoon, the birds were panting and their core body temperatures were significantly higher compared to the morning time, as illustrated by iButton data thermo-loggers. Concomitantly, blood HSP70 mRNA and feather HSP70 expression (mRNA and protein) were significantly increased in the afternoon compared to the morning time. Similarly, HSP70 protein expression in the duodenum was also significantly higher in the afternoon compared to the morning period. Together, these finding identify feather HSP70 as a novel non-invasive molecular signature that mirrors the intracellular and systemic stress, which can be useful to monitor well-being of chickens and other avian species under different challenges.