REGULATION OF ENDOTHELIAL-CELL MORPHOGENESIS BY INTEGRINS, MECHANICAL FORCES, AND MATRIX GUIDANCE PATHWAYS
REGULATION OF ENDOTHELIAL-CELL MORPHOGENESIS BY INTEGRINS, MECHANICAL FORCES, AND MATRIX GUIDANCE PATHWAYS
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DOI：
10.1006/excr.1995.1015
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发表时间：
1995-01-01
影响因子：
3.7
通讯作者：
CAMARILLO, CW
中科院分区：
文献类型：
--
作者：
DAVIS, GE;CAMARILLO, CW
已知基底膜基质诱导人内皮细胞形成索状结构，其模拟在体内早期血管生成期间观察到的索状结构。使用该模型，阻断抗体研究揭示了α 6 β 1整联蛋白在索形成中的主要作用。在该过程中，观察到基质胶结构的两个改变，这表明了索形成精确性的机制。首先，基质胶收缩并从琼脂糖支持物上剥离，其次，在基质胶中可见线性扭曲，其对应于内皮细胞过程的迁移途径。这些途径，我们称之为“基质引导途径”，似乎是由于内皮细胞之间产生机械张力所致。上述数据支持这样的概念，即形态发生事件期间的内皮细胞引导可以通过这些细胞对周围细胞外基质施加机械力以产生迁移途径的能力来控制。(C)出版社：Academic Press
Basement membrane matrix is known to induce human endothelial cells to form cord-like structures that mimic those observed during early angiogenesis in vivo. Using this model, blocking antibody studies revealed a major role for the alpha 6 beta 1 integrin in cord formation, During this process, two alterations in the Matrigel structure were observed which suggested a mechanism for the precision of cord formation. First, Matrigel contracted and lifted off an agarose support and second, linear distortions became visible in the Matrigel that correspond to the migration pathways of endothelial cell processes. These pathways, which we have termed ''matrix guidance pathways,'' appear to result from the generation of mechanical tension between endothelial cells. The above data support the concept that endothelial cell guidance during morphogenetic events could be controlled by the ability of these cells to exert mechanical forces on the surrounding extracellular matrix to create pathways for migration. (C) 1995 Academic Press,Inc.