Sea urchin FGFR muscle-specific expression: posttranscriptional regulation in embryos and adults.
Sea urchin FGFR muscle-specific expression: posttranscriptional regulation in embryos and adults.
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海胆 FGFR 肌肉特异性表达：胚胎和成体的转录后调控。
DOI：
10.1006/dbio.1998.8943
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Angerer,LM
中科院分区：
文献类型：
--
作者：
McCoon,PE;Blackstone,E;Angerer,RC;Angerer,LM
我们已经通过原位杂交表明，在海胆胚胎发生的初始阶段，许多细胞类型中都转录了编码成纤维细胞生长因子受体（SpFGFR）的基因（McCoonet al.， J. Biol.）。化学，2011,31,2011 - 20195,1996)。本研究通过亲和纯化抗体的免疫染色证明，SpFGFR蛋白仅在胚胎的肌肉细胞中可检测到，并且在同一时间出现，这表明其功能与肌肉的命运无关，而是可能需要支持成肌细胞的增殖、迁移和/或分化。令人惊讶的是，我们发现spfgfr转录本在胚胎细胞核中富集，这表明在非肌肉细胞中缺乏加工和/或细胞质运输至少是转录后调节机制的一部分。Western blot结果显示，SpFGFR在成人灯笼肌中也有特异性表达，但在其他平滑肌组织（包括管足和肠）或体腔细胞中未检测到，尽管在所有这些组织中都存在类似浓度的SpFGFR转录本。我们得出结论，在胚胎和成人中，肌肉特异性的SpFGF受体合成主要在转录后水平受到控制。我们通过RNase保护实验表明，编码受体配体结合域的IgS变体的转录本，以前被证明在胚胎内胚层部分中丰富，是主要的，如果不是唯一的，spfgfr转录本。总之，这些结果表明，在成人和胚胎肌肉细胞中，只有少数spfgfr转录本被加工、输出和翻译，这些转录本主要包含（如果不是完全包含）IgS配体结合域序列。
We have shown previously byin situhybridization that a gene encoding a fibroblast growth factor receptor (SpFGFR) is transcribed in many cell types during the initial phases of sea urchin embryogenesis (Strongylocentrotus purpuratus) (McCoonet al., J. Biol. Chem. 271,20119–20195, 1996). Here we demonstrate by immunostaining with affinity-purified antibody that SpFGFR protein is detectable only in muscle cells of the embryo and appears at a time suggesting that its function is not in commitment to a muscle fate, but instead may be required to support the proliferation, migration, and/or differentiation of myoblasts. Surprisingly, we find thatSpFGFRtranscripts are enriched in embryo nuclei, suggesting that lack of processing and/or cytoplasmic transport in nonmuscle cells is at least part of the posttranscriptional regulatory mechanism. Western blots show that SpFGFR is also specifically expressed in adult lantern muscle, but is not detectable in other smooth muscle-containing tissues, including tube foot and intestine, or in coelomocytes, despite the presence ofSpFGFRtranscripts at similar concentrations in all these tissues. We conclude that in both embryos and adults, muscle-specific SpFGF receptor synthesis is controlled primarily at a posttranscriptional level. We show by RNase protection assays that transcripts encoding the IgS variant of the ligand binding domain of the receptor, previously shown to be enriched in embryo endomesoderm fractions, are the predominant, if not exclusive,SpFGFRtranscripts in lantern muscle. Together, these results suggest that only a minority ofSpFGFRtranscripts are processed, exported, and translated in both adult and embryonic muscle cells and these contain predominantly, if not exclusively, IgS ligand binding domain sequences.