Different response to osteo-inductive agents in bone marrow- and periosteum-derived cell preparations.
Different response to osteo-inductive agents in bone marrow- and periosteum-derived cell preparations.
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骨髓和骨膜来源的细胞制剂对骨诱导剂的不同反应。
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
A. Caplan
中科院分区：
文献类型：
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作者：
L. Solchaga;P. Cassiede;A. Caplan
兔骨髓和骨膜来源的细胞培养在培养基中含有地塞米松，骨形态发生蛋白-2（BMP-2）或两者。骨髓来源的细胞的反应，测量碱性磷酸酶的表达，取决于生长阶段。在亚融合培养物中，BMP 2比地塞米松具有更大的作用，并且用两者处理进一步增加酶活性。在融合培养物中，地塞米松的作用大于BMP 2，并且当一起使用时，它们具有累加作用。在这些培养物中观察到的矿物质沉积不具有骨结节的典型结构。对于骨膜来源的细胞，地塞米松并没有增加碱性磷酸酶的表达，而BMP 2，两者的治疗效果不如单独用BMP 2治疗。在用地塞米松和BMP 2处理的骨膜衍生细胞的培养物中观察到典型的骨结节。这些发现表明，来自这两种来源的骨祖细胞本质上是不同的，或者制剂中的非祖细胞直接或间接影响对骨诱导剂的反应性。
Rabbit bone marrow- and periosteum-derived cells were cultured in medium containing dexamethasone, bone morphogenetic protein-2 (BMP2) or both. The response of bone marrow-derived cells, measured as alkaline phosphatase expression, depended on the stage of growth. In subconfluent cultures, BMP2 had a greater effect than dexamethasone and treatment with both further increased enzyme activity. In confluent cultures, the effect of dexamethasone was greater than that of BMP2 and, when used together, they had an additive effect. The mineral deposition observed in these cultures did not have the typical structure of bone nodules. For periosteum-derived cells, dexamethasone did not increase the expression of alkaline phosphatase, while BMP2 did; treatment with both was less effective than treatment with BMP2 alone. Typical bone nodules were observed in cultures of periosteum-derived cells treated with dexamethasone and BMP2. These findings indicate that either osteoprogenitor cells from these two sources are intrinsically different or else non-progenitor cells in the preparations directly or indirectly affect the responsiveness to osteo-inductive agents.
影响因子：
2.7
作者：
RICKARD, DJ;SULLIVAN, TA;KAZHDAN, I
通讯作者：
KAZHDAN, I