Structural properties and bioactivities of pectic polysaccharides isolated from soybean hulls
Structural properties and bioactivities of pectic polysaccharides isolated from soybean hulls
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DOI：
10.1016/j.lwt.2022.114079
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发表时间：
2022-10-13
影响因子：
6
通讯作者：
Liu, He
中科院分区：
文献类型：
--
作者：
Song, Hong;Han, Lin;Liu, He
研究从大豆皮中提取纯化的果胶多糖ASP-3A的结构特征及其抗氧化性。根据高效凝胶渗透色谱、甲基化分析和核磁共振光谱等对ASP-3A的结构和形态进行表征的结果表明，ASP-3A由鼠李糖、半乳糖、半乳糖醛酸、阿拉伯糖、木糖、葡萄糖醛酸、葡萄糖、甘露糖和氨基葡萄糖盐酸盐组成，摩尔比为1.000：0.745：0.570：0.189：0.100。零点一三五：0.098：0.036：0.036：0.008. ASP-3A主要由4）-α-D-GalA β（1 ->）和β-D-Glap-（1 -> 4）-α-L-Rha-（1 ->）组成，分支主要由α-L-Araf-（1 -> 5）-α-L-Araf-（1 ->）组成，表明ASP-3A是一种含有高半乳糖醛酸聚糖（HG）和鼠李糖半乳糖醛酸聚糖I（RG-I）结构域的果胶多糖。ASP-3A在超氧阴离子自由基、羟基自由基、ABTS中心点+和DPPH中心点清除试验中表现出剂量依赖性抗氧化活性，SC 50值分别为1.083 +/- 0.060、2.507 +/- 0.387、1.677 +/- 0.027和1.325 +/- 0.002 mg/mL。ASP-3A通过NRF 2/ARE途径增强肝细胞抗氧化能力，减轻CCl 4诱导的氧化应激。本研究为大豆皮中天然多糖类抗氧化剂的筛选提供了依据。
The purpose of this study was to investigate the structural characteristic and its antioxidant of a pectic polysaccharide (ASP-3A) extracted and purified from soybean hulls. Results of structural and morphological characterization according to high-performance gel-permeation chromatography, methylation analysis and nuclear magnetic resonance spectroscopy etc. showed that ASP-3A was composed of rhamnose, galactose, galacturonic acid, arabinose, xylose, glucuronic acid, glucose, mannose, and glucosamine hydrochloride with molar ratios of 1.000 : 0.745: 0.570 : 0.189: 0.135 : 0.098: 0.036 : 0.036: 0.008. ASP-3A was mainly consisted of 4)-alpha-D-GalAp(1 -> and beta-D-Glap-(1 -> 4)-alpha-L-Rha-(1 ->, with branch consisting principally of alpha-L-Araf-(1 -> 5)-alpha-L-Araf-(1 ->, which suggested that ASP-3A was a pectin polysaccharide including homogalacturonan (HG) and rhamnogalacturonan I (RG-I) domain. ASP-3A exhibited dose-dependent antioxidant activities in superoxide anion radical, hydroxy radical, ABTS center dot+ and DPPH center dot scavenging assays with SC50 values of 1.083 +/- 0.060, 2.507 +/- 0.387, 1.677 +/- 0.027 and 1.325 +/- 0.002 mg/mL, respectively. ASP-3A alleviated oxidative stress induced by CCl4 through enhancing antioxidant capacity of hepatocytes via NRF2/ARE pathway. This study provides information useful for screening natural polysaccharide-based antioxidants in soybean hulls.