Comparative physiological and proteomic analyses reveal the actions of melatonin in the reduction of oxidative stress in Bermuda grass (Cynodon dactylon (L). Pers.)
Comparative physiological and proteomic analyses reveal the actions of melatonin in the reduction of oxidative stress in Bermuda grass (Cynodon dactylon (L). Pers.)
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DOI：
10.1111/jpi.12246
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发表时间：
2015-08-01
影响因子：
10.3
通讯作者：
Chan, Zhulong
中科院分区：
文献类型：
--
作者：
Shi, Haitao;Wang, Xin;Chan, Zhulong
褪黑素在动物中是一种重要的抗氧化剂，这一事实使植物研究人员推测，褪黑素在植物中也以类似的方式起作用。虽然褪黑激素在缓解应激触发的活性氧（ROS）方面有显著作用，但褪黑激素在植物中直接参与氧化应激及其潜在的生理和分子机制尚不清楚。在本研究中，我们发现外源褪黑激素显著缓解了过氧化氢（H2O2）调节的百慕大草植物生长、细胞损伤和ROS积累。此外，使用iTRAQ（相对和绝对定量等压标记），通过无凝胶蛋白质组学鉴定了76个在模拟或H2O2处理期间受褪黑激素显著影响的蛋白质。代谢途径分析表明，褪黑素和H2O2处理显著增强了多胺代谢、核糖体代谢、主要碳水化合物代谢、光合作用、氧化还原和氨基酸代谢等途径。综上所述，本研究为百慕大草对直接氧化应激反应中褪黑素的生理和分子机制提供了更全面的见解。这可能与抗氧化剂的激活、代谢途径的调节和广泛的蛋白质组重编程有关。
The fact of melatonin as an important antioxidant in animals led plant researchers to speculate that melatonin also acts in the similar manner in plants. Although melatonin has significant effects on alleviating stress-triggered reactive oxygen species (ROS), the involvement of melatonin in direct oxidative stress and the underlying physiological and molecular mechanisms remain unclear in plants. In this study, we found that exogenous melatonin significantly alleviated hydrogen peroxide (H2O2)-modulated plant growth, cell damage, and ROS accumulation in Bermuda grass. Additionally, 76 proteins significantly influenced by melatonin during mock or H2O2 treatment were identified by gel-free proteomics using iTRAQ (isobaric tags for relative and absolute quantitation). Metabolic pathway analysis showed that several pathways were markedly enhanced by melatonin and H2O2 treatments, including polyamine metabolism, ribosome pathway, major carbohydrate metabolism, photosynthesis, redox, and amino acid metabolism. Taken together, this study provides more comprehensive insights into the physiological and molecular mechanisms of melatonin in Bermuda grass responses to direct oxidative stress. This may relate to the activation of antioxidants, modulation of metabolic pathways, and extensive proteome reprograming.