Spatiotemporal Control of Genetic Circuit with Pulse Generation for Molecular Communication
Spatiotemporal Control of Genetic Circuit with Pulse Generation for Molecular Communication
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DOI：
10.1109/globecom46510.2021.9685659
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发表时间：
2021-12
期刊：
影响因子：
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通讯作者：
Dadi Bi;Yansha Deng
中科院分区：
文献类型：
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作者：
Dadi Bi;Yansha Deng
合成生物学提供了一种工具来构建生物实体，这些生物实体能够通过控制和工程化生化信号传导途径来执行所需的信号处理功能。表现出自然行为的遗传电路的设计和研究可以有助于更好地理解基因表达行为背后的原理和动力学，以及用于合成生物学的工程细胞系统。在本文中，我们提出了一个合成系统，能够产生脉冲形状的信号，这是一个普遍的行为，在自然环境中。特别是，我们提出的系统是基于一个工程细胞与NOR逻辑操作，脉冲产生利用的NOR输入的逻辑状态的改变，由两种类型的扩散信号分子控制。为了定量描述所产生的脉冲，我们不仅推导出信号分子的传播通道，而且使用Shea-Ackers形式主义分析工程细胞的行为。仿真结果表明，脉冲整形信号可以成功地产生在一个可控的方式。
Synthetic biology offers a tool to build biological entities that are capable of carrying out desired signal processing functionalities by controlling and engineering biochemical signaling pathways. The design and study of genetic circuits that exhibit natural behavior can be helpful for an improved understanding of the principles and kinetics behind the gene expression behavior, as well as for engineering cellular systems for synthetic biology. In this paper, we propose a synthetic system capable of generating a pulse-shaped signal, which is a prevalent behavior in natural environment. In particular, our proposed system is based on an engineered cell with NOR logic operation, and the pulse generation exploits the alteration in logic states of NOR inputs that are controlled by two types of diffusive signaling molecules. To quantitatively describe the generated pulse, we not only derive the propagation channel of the signaling molecules but also analyze the behavior of the engineered cell using Shea-Ackers formalism. Simulation results demonstrate that the pulse-shaped signal can be successfully produced in a controllable manner.