Increasing the efficiency of the plate counting method for estimating bacterial diversity
Increasing the efficiency of the plate counting method for estimating bacterial diversity
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DOI：
10.1016/s0167-7012(97)00056-0
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发表时间：
1997-08-01
影响因子：
2.2
通讯作者：
Misaghi, IJ
中科院分区：
文献类型：
--
作者：
Balestra, GM;Misaghi, IJ
一组25种不同的培养基，选择他们的能力，以支持大量的细菌的生长，产生四到七倍之间的形态不同的细菌从黑麦草和苜蓿根和土壤样品时，用于平板计数比单一的培养基。选择的25种培养基包括一些常用的培养基，补充了抗生素或有毒物质，以及仅含有单一营养素的培养基。单独使用的培养基包括King's B、Luria-Bertani、营养琼脂、胰蛋白酶大豆琼脂和冷提取土壤提取物琼脂。根据本研究的结果，我们建议使用一组不同的培养基，而不是一种或两种培养基，以提高平板计数法估计细菌多样性的效率。(C)1997年Elsevier Science B.V.
A set of 25 different media, selected for their ability to support the growth of a large number of bacteria, yielded between four to seven times more morphologically distinct bacteria from ryegrass and alfalfa roots and soil samples when used for plate counting than a single medium. The 25 media in the selection included some of the commonly used media supplemented with antibiotics or toxicants and media containing only a single nutrient. Media used singly included King's B, Luria-Bertani, Nutrient Agar, Tryptic Soy Agar, and Cold-extracted Soil Extract Agar. Based on the results of this study we recommend the use of a set of different media, as opposed to one or two media, in order to increase the efficiency of plate counting method for estimating bacterial diversity. (C) 1997 Elsevier Science B.V.