Interspecies implantation and mitochondria fate of panda-rabbit cloned embryos
Interspecies implantation and mitochondria fate of panda-rabbit cloned embryos
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DOI：
10.1095/biolreprod67.2.637
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发表时间：
2002-08-01
影响因子：
3.6
通讯作者：
Zhang, HM
中科院分区：
文献类型：
--
作者：
Chen, DY;Wen, DC;Zhang, HM
大熊猫的体细胞核在去核兔卵质中可以去分化，重建的卵可以发育成囊胚。为了观察这些跨物种克隆胚胎是否可以植入大熊猫以外的动物子宫，我们将2300个熊猫-兔子克隆胚胎移植到100个同步的兔子受体中，没有一个成功怀孕。在另一种方法中，我们将熊猫-兔子和猫-兔子的种间克隆胚胎共同移植到21只猫的输卵管中。14例受者在35天内出现发情；5例受者在胚胎移植后43 ~ 48天出现发情；另外两名接受者死于肺炎，其中一名在尸检时被发现怀了六个早期胎儿。对这些早期胎儿的微卫星DNA分析证实，其中两个来自大熊猫-兔子克隆胚胎。结果表明，熊猫-兔子克隆胚胎可以植入第三种物种——家猫的子宫。利用熊猫和兔的线粒体特异性探针，我们发现在胚胎着床后早期胚胎中，来自熊猫体细胞和兔卵浆的线粒体共存，但来自兔卵浆的线粒体减少，而来自熊猫供体细胞的线粒体占主导地位。我们的研究结果表明，在移植后的种间克隆胚胎中，来自供体细胞的线粒体可以替代来自受体卵母细胞的线粒体。
Somatic cell nuclei of giant pandas can dedifferentiate in enucleated rabbit ooplasm, and the reconstructed eggs can develop to blastocysts. In order to observe whether these interspecies cloned embryos can implant in the uterus of an animal other than the panda, we transferred approximately 2300 panda-rabbit cloned embryos into 100 synchronized rabbit recipients, and none became pregnant. In another approach, we co-transferred both panda-rabbit and cat-rabbit interspecies cloned embryos into the oviducts of 21 cat recipients. Fourteen recipients exhibited estrus within 35 days; five recipients exhibited estrus 43-48 days after embryo transfer; and the other two recipients died of pneumonia, one of which was found to be pregnant with six early fetuses when an autopsy was performed. Microsatellite DNA analysis of these early fetuses confirmed that two were from giant panda-rabbit cloned embryos. The results demonstrated that panda-rabbit cloned embryos can implant in the uterus of a third species, the domestic cat. By using mitochondrial-specific probes of panda and rabbit, we found that mitochondria from both panda somatic cells and rabbit ooplasm coexisted in early blastocysts, but mitochondria from rabbit ooplasm decreased, and those from panda donor cells dominated in early fetuses after implantation. Our results reveal that mitochondria from donor cells may substitute those from recipient oocytes in postimplanted, interspecies cloned embryos.