The spore photoproduct lyase repairs the 5S- and not the 5R-configured spore photoproduct DNA lesion
The spore photoproduct lyase repairs the 5S- and not the 5R-configured spore photoproduct DNA lesion
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DOI：
10.1039/b514103f
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发表时间：
2006-01-28
影响因子：
4.9
通讯作者：
Carell, T
中科院分区：
文献类型：
--
作者：
Friedel, MG;Berteau, O;Carell, T
孢子光产物裂解酶是一种Fe-S/CuMet DNA修复酶，其使用未知的自由基机制直接修复由孢子的UV照射诱导的孢子损伤。空气敏感的自由基SAM酶的第一次挑战与合成纯底物。发现该酶识别合成的5S构型的孢子损伤，而没有中心磷酸二酯键。5 R配置的病变与目前的信念相反，不是基底。
The spore photoproduct lyase is a Fe-S/AdoMet DNA repair enzyme, which directly repairs spore lesions, induced by UV irradiation of spores, using an unknown radical mechanism. The air sensitive radical SAM enzyme was for the first time challenged with synthetically pure substrates. It was found that the enzyme recognizes a synthetic 5S-configured spore lesion without the central phosphodiester bond. The 5R-configured lesion is in contrast to current belief not a substrate.