Application of intra- and extracellular sugars and dimethylsulfoxide to human oocyte cryopreservation.
Application of intra- and extracellular sugars and dimethylsulfoxide to human oocyte cryopreservation.
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细胞内外糖和二甲基亚砜在人卵母细胞冷冻保存中的应用。
DOI：
10.1007/s10815-009-9316-8
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发表时间：
2009
影响因子：
3.1
通讯作者：
Eroglu,Ali
中科院分区：
文献类型：
--
作者：
Younis,Abdelmoneim;Carnovale,David;Butler,William;Eroglu,Ali
目的卵母细胞冷冻可以避免胚胎冷冻的许多并发症，为接受癌症治疗的妇女提供未来的生育能力。本研究的目的是探索细胞内和细胞外糖与少量二甲亚砜（DMSO）结合在人卵母细胞冷冻保存中的应用，作为一种替代方法。方法：经知情同意和IRB批准的体外受精（IVF）患者的丢弃人卵母细胞，随机分为三组：(i)不加细胞内和细胞外糖的DMSO对照组；（ii）细胞外糖（棉子糖）+ DMSO；（iii）细胞内和细胞外糖（海藻糖和棉子糖分别）+ DMSO。随后，将所有冷冻保存的卵母细胞快速解冻，培养24 h后以形态学标准评估其存活。结果三组共71只卵母细胞，细胞内糖+DMSO、细胞外糖+DMSO和DMSO对照组的存活率分别为88.5%（23/26）、68.2%（15/22）和52.2%（12/23）。结论细胞内糖和细胞外糖可作为人类卵母细胞低温保存的替代方法。
PurposeOocyte cryopreservation may avoid many complications of human embryo freezing and provide future fertility for women undergoing cancer therapy. The objective of this study was to explore the application of intra- and extracellular sugars in combination with small amounts of dimethylsulfoxide (DMSO) to human oocyte cryopreservation as an alternative approach.MethodsDiscarded human oocytes that were obtained from IVF patients under informed consent and IRB approval, were cryopreserved by slow cooling to −196°C after being randomly distributed into three groups: (i) DMSO control without intra- and extracellular sugar; (ii) extracellular sugar (raffinose) + DMSO; and (iii) intra- and extracellular sugar (trehalose and raffinose, respectively) + DMSO. Subsequently, all cryopreserved oocytes were thawed rapidly, and their survival was assessed by morphological criteria after 24 h of culture.ResultsA total of 71 oocytes were evaluated in three groups with survival rates of 88.5% (23/26), 68.2% (15/22), and 52.2% (12/23) for intra- and extracellular sugar+DMSO, extracellular sugar+DMSO, and DMSO control groups, respectively.ConclusionThese results support the use of intra- and extracellular sugars as an alternative approach for cryopreservation of human oocytes.