punt and schnurri regulate a somatically derived signal that restricts proliferation of committed progenitors in the germline.
punt and schnurri regulate a somatically derived signal that restricts proliferation of committed progenitors in the germline.
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发表时间：
1997-11
期刊：
影响因子：
4.6
通讯作者：
E. Matunis;John Tran;Pierre Gönczy;Kim Caldwell;Stephen DiNardo
中科院分区：
文献类型：
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作者：
E. Matunis;John Tran;Pierre Gönczy;Kim Caldwell;Stephen DiNardo
为了确定干细胞谱系的调节者，我们将重点放在果蝇的精子发生上。在精子发生过程中，每个生殖系干细胞不对称地分裂，自我更新并产生一个瞬时放大的子代，该子代分裂四次。通过筛选子代细胞无法停止分裂的突变，我们发现转化生长因子-β信号转导蛋白SchNurri和Punt是限制生殖细胞瞬时扩增所必需的。马赛克分析表明，Punt和SchNurri在生殖细胞周围的体细胞囊细胞内发挥作用，而不是在生殖细胞内。因此，囊细胞来源的信号限制生殖细胞的增殖，该信号是由转化生长因子-β超家族成员的输入启动的。因此，信号传递调节生殖系干细胞谱系的进展。
To identify regulators of stem cell lineages, we are focusing on spermatogenesis in Drosophila. In spermatogenesis, each germline stem cell divides asymmetrically, renewing itself and producing a transiently amplifying daughter, which divides four times. By screening for mutants in which daughter cells fail to stop dividing, we find that the TGF-beta signal transducers schnurri and punt are required to limit transient amplification of germ cells. Mosaic analysis demonstrates that punt and schnurri act within somatic cyst cells that surround germ cells, rather than in germ cells. Thus, a cyst-cell-derived signal restricts germ cell proliferation and this signal is initiated by input from a member of the TGF-beta superfamily. Thus, a signal relay regulates progression through the germline stem cell lineage.