Annexin II, a Novel HSP27-interacted Protein, is Involved in Resistance to UVC-induced Cell Death in Human APr-1 Cells
Annexin II, a Novel HSP27-interacted Protein, is Involved in Resistance to UVC-induced Cell Death in Human APr-1 Cells
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DOI：
10.1111/j.1751-1097.2008.00365.x
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发表时间：
2008-11-01
影响因子：
3.3
通讯作者：
Suzuki, Nobuo
中科院分区：
文献类型：
--
作者：
Tong, Xiao-Bo;Kita, Kazuko;Suzuki, Nobuo
热休克蛋白27（HSP 27）作为一种分子伴侣参与多种生物学功能。我们发现HSP 27参与保护人类细胞免受UVC致死。为了阐明抗UVC的分子机制，我们在抗UVC的人类细胞AP（r）-1中寻找与抗性相关的HSP 27相互作用蛋白。用GST-融合的HSP 27蛋白结合亲和层析柱从细胞裂解液中筛选出3个HSP 27相互作用的候选蛋白。通过免疫沉淀分析证实了HSP 27与两个候选物膜联蛋白II和HSP 70之间的相互作用。UVC照射后，HSP 27和膜联蛋白II的复合物的量在核后部分减少，而在核部分增加。用膜联蛋白II-siRNA转染的细胞对UVC致死性更敏感。这些结果表明，膜联蛋白II是一种新的HSP 27相互作用的蛋白质，参与人体细胞的UVC抗性，至少在这里测试的那些。
Heat shock protein 27 (HSP27) is implicated in diverse biologic functions as a molecular chaperone. We found that HSP27 is involved in the protection of human cells against UVC lethality. To elucidate the molecular mechanisms underlying UVC resistance, we searched for HSP27-interacted proteins related to resistance in UVC-resistant human cells, AP(r)-1. Three candidates for HSP27-interacted proteins were found from cell lysates using an affinity column coupled with GST-fused HSP27 protein. Interaction between HSP27 and two candidates, annexin II and HSP70, was confirmed by immunoprecipitation analysis. After UVC irradiation, the amount of the complex of HSP27 and annexin II decreased in the postnuclear fraction, while it increased in the nuclear fraction. Cells transfected with annexin II-siRNA were more susceptible to UVC lethality. These results suggest that annexin II is a novel HSP27-interacted protein which is involved in UVC resistance in human cells, at least those tested here.