Plasmacytoid dendritic cells and memory T cells infiltrate true sequestrations stronger than subligamentous sequestrations: evidence from flow cytometric analysis of disc infiltrates
Plasmacytoid dendritic cells and memory T cells infiltrate true sequestrations stronger than subligamentous sequestrations: evidence from flow cytometric analysis of disc infiltrates
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DOI：
10.1007/s00586-015-4325-z
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发表时间：
2016-05-01
影响因子：
2.8
通讯作者：
Eysel, Peer
中科院分区：
文献类型：
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作者：
Geiss, Andrea;Sobottke, Rolf;Eysel, Peer
目的髓核突出被认为可以诱导获得性免疫反应。抗原呈递细胞（APC）的抗原识别代表了适应性免疫应答表现中的重要步骤。巨噬细胞已被假定为APC的功能，而浆细胞样树突状细胞的重要性，启动针对突出的髓核的免疫反应从来没有被检查。本研究的目的是评估浆细胞样树突状细胞的重要性，启动针对突出discs.Methods的免疫反应15例真正的隔离和3例subligamycin隔离进行手术后，他们的神经系统检查。收集椎间盘材料，称重并消化90 min。对分离的单细胞进行计数，对浆细胞样树突状细胞（CD123（+）CD4（+））、巨噬细胞（CD14（+）CD11c（+））和记忆T细胞（CD4（+）CD45RO（+））进行染色，并通过流式细胞术进行分析。比较两组患者的细胞比例。此外，根据肌无力的严重程度和直腿抬高（SLR）试验结果，将真性隔离症患者（TRUE患者）细分为亚组。结果浆细胞样树突状细胞和记忆T细胞对真隔离区的浸润强于升华隔离区，且在真隔离区中浆细胞样树突状细胞比巨噬细胞占主导地位。最高比例的浆细胞样树突状细胞中检测到浸润的患者有真正的隔离，严重的肌无力和阴性结果在SLR test.Conclusions本研究的结果表明，浆细胞样树突状细胞参与启动针对突出的髓核的免疫反应，而巨噬细胞可能会加强表现出的免疫反应和介导椎间盘吸收。
Purpose Herniated nucleus pulposus has been considered to induce an adaptive immune response. Antigen recognition by antigen-presenting-cells (APCs) represents an important step within manifestation of an adaptive immune response. Macrophages have been assumed to function as APC, while importance of plasmacytoid dendritic cells for initiation of an immune response directed towards herniated nucleus pulposus has never been examined. The aim of the present study was to assess importance of plasmacytoid dendritic cells for initiation of immune response directed towards herniated discs.Methods Fifteen patients with true sequestrations and three patients with subligamentous sequestrations underwent surgery after their neurological examinations. Disc material was harvested, weighted and digested for 90 min. Separated single cells were counted, stained for plasmacytoid dendritic cells (CD123(+) CD4(+)), macrophages (CD14(+) CD11c(+)) and memory T cells (CD4(+) CD45RO(+)) and analysed by flow cytometry. Both patient groups were compared in cell proportions. Furthermore, patients with true sequestrations (TRUE patients) were subdivided into subgroups based on severity of muscle weakness and results in straight leg raising (SLR) test. Subgroups were compared in cell proportions.Results Plasmacytoid dendritic cells and memory T cells infiltrated true sequestrations stronger than the subligamentous sequestration and plasmacytoid dendritic cells predominated over macrophages in true sequestrations. Highest proportions of plasmacytoid dendritic cells were detected in infiltrates of patients having true sequestrations, severe muscle weakness and negative result in SLR test.Conclusions The findings of the present study indicate that plasmacytoid dendritic cells are involved in initiation of an immune response directed towards herniated nucleus pulposus, while macrophages may reinforce the manifested immune response and mediate disc resorption.