Analysis of telomere length and telomerase activity in tree species of various life-spans, and with age in the bristlecone pine Pinus longaeva
Analysis of telomere length and telomerase activity in tree species of various life-spans, and with age in the bristlecone pine Pinus longaeva
复制标题
DOI：
10.1007/s10522-005-3484-4
复制
发表时间：
2005-03-01
期刊：
影响因子：
4.5
通讯作者：
Kletetschka, G
中科院分区：
文献类型：
--
作者：
Flanary, BE;Kletetschka, G
正常的体细胞具有有限的复制能力。随着每次细胞分裂，端粒（线性染色体的物理末端）逐渐缩短，直到达到临界长度，此时细胞进入复制衰老。一些细胞通过端粒酶的作用保持端粒的长度。刺果松（Pinus longaeva）是已知最古老的真核生物，2005年有记录的最古老的真核生物已经4770岁了。为了确定端粒长度和端粒酶活性随年龄的变化，以及端粒长度和端粒酶活性在延长长寿树的寿命和寿命方面所起的作用，以及在其他不同寿命的树种中所起的作用，我们对这些树木的端粒长度和端粒酶活性进行了详细的调查。这项研究的结果支持了这样一个假设，即端粒长度的增加和端粒酶活性的增加可能直接/间接地有助于长寿松树的寿命和寿命的增加（2000-5000年寿命）与中等寿命相比（400-500年寿命）和短命（100-200年寿命）松树，以及在P. longaeva随着年龄的增长。
Normal somatic cells have a finite replicative capacity. With each cell division, telomeres (the physical ends of linear chromosomes) progressively shorten until they reach a critical length, at which point the cells enter replicative senescence. Some cells maintain telomere length by the action of the telomerase enzyme. The bristlecone pine, Pinus longaeva, is the oldest known living eukaryotic organism, with the oldest on record turning 4770 years old in 2005. To determine what changes occur, if any, in telomere length and telomerase activity with age, and what roles, if any, telomere length and telomerase activity may play in contributing to the increased life-span and longevity of P. longaeva with age, as well as in other tree species of various life-spans, we undertook a detailed investigation of telomere length and telomerase activity in such trees. The results from this study support the hypothesis that both increased telomere length and telomerase activity may directly/indirectly contribute to the increased life-span and longevity evident in long-lived pine trees (2000-5000 year life-spans) compared to medium-lived (400-500 year life-span) and short-lived (100-200 year life-span) pine trees, as well as in P. longaeva with age.