A new model of posterior capsule opacification in rodents
A new model of posterior capsule opacification in rodents
复制标题
DOI：
10.1167/iovs.02-1293
复制
发表时间：
2003-08-01
影响因子：
4.4
通讯作者：
Forrester, JV
中科院分区：
文献类型：
--
作者：
Lois, N;Dawson, R;Forrester, JV
目的。描述一种啮齿类动物后囊膜混浊（PCO）的新模型。对42只褐挪威大鼠进行连续曲线囊裂和水解剖的囊外晶状体摘除（ECLE）。分别于术后0、6、24小时、3、7、14天处死。取眼球去核，进行光镜和免疫组织化学处理。34只（81%）动物手术后死前眼睛愈合良好，角膜清晰，前房形成良好。8例（19%）患者由于前房积血、纤维蛋白或角膜混浊而未见前段结构。在ECLE后3天出现PCO，所有动物在2周时均出现PCO。ECLE后，晶状体上皮细胞（LECs）出现在前囊和晶状体弓的内表面。术后24小时，lec开始向后囊中心移动。第3天，晶状体囊内出现了多层的晶状体细胞，有些呈纺锤形。包膜明显起皱。术后14天，所有动物均观察到晶状体纤维和索默林氏环，表明细胞有一定程度的分化。术后第3天和第14天活化的巨噬细胞数量较多（P < 0.05），此时lec的增殖和迁移最大，晶状体纤维分化明显。在啮齿类动物中，PCO发生在ECLE后，并与低级别炎症有关，主要是单核巨噬细胞。虽然没有进行人工晶状体植入术，但该模型对于研究白内障手术后导致PCO的事件序列和促进晶状体纤维分化的细胞外基质线索似乎很有价值。
PURPOSE. To describe a new model of posterior capsule opacification (PCO) in rodentsMETHODS. An extracapsular lens extraction (ECLE), by continuous curvilinear capsulorrhexis and hydrodissection, was performed in 42 consecutive Brown Norway rats. Animals were killed at 0, 6, and 24 hours and 3, 7, and 14 days after surgery. Eyes were enucleated and processed for light microscopy and immunohistochemistry.RESULTS. In 34 (81%) of the animals the operated eye appeared well healed before death, with a clear cornea and a well-formed anterior chamber. In eight (19%) there was no view of anterior segment structures because of hyphema, fibrin, or corneal opacification. PCO was clinically evident 3 days after ECLE and was present in all animals at 2 weeks. Immediately after ECLE, lens epithelial cells (LECs) were present in the inner surface of the anterior capsule and lens bow. Twenty-four hours after surgery, LECs started to migrate toward the center of the posterior capsule. At 3 days, multilayered LECs, some spindle shaped, were present throughout the lens capsule. Capsular wrinkling was apparent. Lens fibers and Soemmering's ring were observed in all animals 14 days after surgery, indicating some degree of cellular differentiation. Activated macrophages were present in greater numbers at 3 and 14 days after surgery (P < 0.05), when proliferation and migration of LECs appeared to be greatest, and lens fiber differentiation was evident, respectively.CONCLUSIONS. In rodents PCO occurs after ECLE and is associated with low-grade inflammation, mostly of mononuclear macrophages. Although no intraocular lens implantation was performed, this model appears to be valuable for studying the sequence of events that leads to PCO after cataract surgery and the extracellular matrix cues that promote lens fiber differentiation.