Interaction and oxidative damage of DVDMS to BSA: a study on the mechanism of photodynamic therapy-induced cell death.
Interaction and oxidative damage of DVDMS to BSA: a study on the mechanism of photodynamic therapy-induced cell death.
复制标题
DVDMS与BSA的相互作用和氧化损伤：光动力疗法诱导细胞死亡的机制研究
DOI：
10.1038/srep43324
复制
发表时间：
2017-03-02
影响因子：
4.6
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Li L;Wang H;Wang H;Li L;Wang P;Wang X;Liu Q
光动力学疗法（PDT）是治疗肿瘤和非肿瘤性疾病的一种很有前途的方法。本研究以中卟啉钠（DVDMS）作为光敏剂，结合光照，研究其对不同细胞系的细胞毒作用。为此，我们选择牛血清白蛋白（BSA）作为模型，从分子水平探讨PDT诱导细胞死亡的机制。我们的研究结果表明，联合治疗显着抑制细胞存活。荧光光谱显示DVDMS与BSA分子在水溶液中有很强的相互作用，影响DVDMS的靶向分布和代谢。光谱分析和羰基含量检测表明，在相似的实验条件下，DVDMS-PDT比Photofrin II-PDT对BSA的损伤程度更高。我们的观察结果与细胞毒性结果一致。光敏剂与光的协同作用可诱导机体产生过量的活性氧（ROS），对BSA和肿瘤细胞的损伤起重要作用。这些结果表明，DVDMS与蛋白质分子的相互作用和氧化损伤是导致细胞死亡的主要原因，为今后的DVDMS-PDT研究提供了有价值的参考。
Photodynamic therapy (PDT) is a promising method for neoplastic and nonneoplastic diseases. In this study, we utilized sinoporphyrin sodium (DVDMS) as a sensitizer combined with light to investigate its cytotoxic effect on different cell lines. For this purpose, we chose bovine serum albumin (BSA) as a model to explore the mechanism of PDT-induced cell death at a molecular level. Our findings indicated that the combined treatment significantly suppressed cell survival. Fluorescence spectroscopy revealed a strong interaction between DVDMS and BSA molecules in aqueous solution, affecting DVDMS’ targeting distribution and metabolism. Spectroscopic analysis and carbonyl content detection indicated that DVDMS-PDT significantly enhanced the damage of BSA at a higher extent than Photofrin II-PDT under similar experimental conditions. Our observations were consistent with the cytotoxicity results. Excessive reactive oxygen species (ROS) were induced by the synergy effect of the sensitizer and light, which played an important role in damaging BSA and tumor cells. These results suggested that the interaction and oxidative damage of protein molecules by DVDMS were the main reasons to cell death and constitute a valuable reference for future DVDMS-PDT investigations.