Heme protein identified from scaly-foot gastropod can synthesize pyrite (FeS2) nanoparticles
Heme protein identified from scaly-foot gastropod can synthesize pyrite (FeS2) nanoparticles
复制标题
从鳞足腹足动物中鉴定出的血红素蛋白可以合成黄铁矿（FeS2）纳米颗粒
DOI：
10.1016/j.actbio.2023.03.005
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发表时间：
2023
影响因子：
9.7
通讯作者：
Suzuki Yoh
中科院分区：
文献类型：
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作者：
Yamashita Tatsuya;Matsuda Hiroki;Koizumi Kyohei;Thirumalaisamy Logu;Kim Myeongok;Negishi Lumi;Kurumizaka Hitoshi;Tominaga Yoriko;Takagi Yoshihiro;Takai Ken;Okumura Taiga;Katayama Hidekazu;Horitani Masaki;Ahsan Nazmul;Okada Yoshitaka;Nagata Koji;Suzuki Yoh
鳞足腹足动物（学名：Eschomallon squamiferum）生活在深海热液喷口附近，有黑色的外壳和足上的鳞片。黑壳和鳞片都含有硫化铁矿物，如硫铁矿（Fe 3S 4）和黄铁矿（FeS 2）。虽然黄铁矿纳米粒子可以作为太阳能电池板的材料，但在体外合成稳定的球形纳米粒子是困难的。在这项研究中，我们提取的有机分子与纳米黄铁矿的鳞足腹足动物的外壳，开发一种低成本，生态友好的方法合成黄铁矿纳米粒子。肌红蛋白（csMG），血红素蛋白，被确定在壳的硫化铁层。我们纯化了重组csMG（r-csMG），并证明r-csMG有助于在80°C下将铁离子、硫离子和硫转化为球形黄铁矿纳米颗粒。为了减少生产的工作量和成本，我们发现商业上可获得的马肌红蛋白（ecMG）也诱导了黄铁矿纳米颗粒的体外合成。使用消化肽的结构-功能实验，我们强调了r-csMG肽的氨基酸序列控制纳米颗粒的球形形状，而与肽相互作用的氯化血红素分子保持纳米颗粒的大小。合成的黄铁矿纳米粒子在可见光区表现出很强的光致发光，表明其作为光伏太阳能电池材料的潜在应用。这些结果表明，用于太阳能电池的材料可以在低成本和能源的环境友好的conditions.Statement的significancePyrite是一个非常有前途的光伏器件材料，因为它具有优良的光学，电学，磁学，和输运性能和高的光吸收系数。目前几乎所有的硫铁矿合成方法都是在高温高压和还原条件下使用有机溶剂。合成的黄铁矿纳米颗粒是不稳定的，并且难以在设备中使用。鳞足腹足类在体内可以合成纳米黄铁矿颗粒，这意味着在低温水环境中可以合成纳米黄铁矿颗粒。在这项研究中，我们证明了黄铁矿纳米粒子的合成使用血红素蛋白质中确定的硫化铁层的鳞足腹足类外壳。这些结果说明了生物体中的天然产物如何激发新技术的创新。
The scaly-foot gastropod (Chrysomallon squamiferum), which lives in the deep-sea zone of oceans around thermal vents, has a black shell and scales on the foot. Both the black shell and scales contain iron sulfide minerals such as greigite (Fe3S4) and pyrite (FeS2). Although pyrite nanoparticles can be used as materials for solar panels, it is difficult to synthesize stable and spherical nanoparticlesin vitro. In this study, we extracted organic molecules that interact with nano-pyrite from the shell of the scaly-foot gastropod to develop a low-cost, eco-friendly method for pyrite nanoparticles synthesis. Myoglobin (csMG), a heme protein, was identified in the iron sulfide layer of the shell. We purified recombinant csMG (r-csMG) and demonstrated that r-csMG helped in the conversion of ferric ions, sulfide ions and sulfur into spherical shaped pyrite nanoparticles at 80°C. To reduce the effort and cost of production, we showed that commercially available myoglobin fromEquus caballus(ecMG) also induced thein vitrosynthesis of pyrite nanoparticles. Using structure-function experiments with digested peptides, we highlighted that the amino acid sequence of r-csMG peptides controlled the spherical shape of the nanoparticle while the hemin molecules, which the peptides interacted with, maintained the size of nanoparticles. Synthesized pyrite nanoparticles exhibited strong photoluminescence in the visible wavelength region, suggesting its potential application as a photovoltaic solar cell material. These results suggest that materials for solar cells can be produced at low cost and energy under eco-friendly conditions.Statement of significancePyrite is a highly promising material for photovoltaic devices because of its excellent optical, electrical, magnetic, and transport properties and high optical absorption coefficient. Almost all current pyrite synthesis methods use organic solvents at high temperature and pressure under reducing conditions. Synthesized pyrite nanoparticles are unstable and are difficult to use in devices. The scaly-foot gastropod can synthesize pyrite nanoparticlesin vivo,meaning that pyrite nanoparticles can be generated in an aqueous environment at low temperature. In this study, we demonstrated the synthesis of pyrite nanoparticles using a heme protein identified in the iron sulfide layer of the scaly-foot gastropod shell. These results exemplify how natural products in organisms can inspire the innovation of new technology.