Tyrosine kinase LYN is an oncotarget in human cervical cancer: A quantitative proteomic based study.
Tyrosine kinase LYN is an oncotarget in human cervical cancer: A quantitative proteomic based study.
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DOI：
10.18632/oncotarget.12258
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发表时间：
2016-11-15
期刊：
影响因子：
--
通讯作者：
Hu L
中科院分区：
文献类型：
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作者：
Liu S;Hao X;Ouyang X;Dong X;Yang Y;Yu T;Hu J;Hu L
宫颈癌是女性最常见的恶性肿瘤之一。宫颈癌的发病机制错综复杂，尚未完全了解。因此，我们采用iTRAQ获得新的蛋白质谱参与宫颈癌的肿瘤发生。共检测出3300个蛋白在宫颈癌组织中的异常表达，并通过western blot验证iTRAQ的结果。然后，我们选择林恩进行进一步研究。免疫组化结果显示，林恩在宫颈癌组织中的表达明显高于癌旁正常宫颈组织和正常宫颈组织。林恩表达的增加与癌症分化和FIGO分期显着相关。沉默林恩可抑制细胞增殖、迁移和侵袭，过表达林恩可促进细胞增殖、迁移和侵袭。在机制上，林恩还可能通过激活IL-6/STAT 3通路促进宫颈癌细胞的转移。体内实验表明，过表达林恩促进肿瘤生长，而敲低林恩则抑制肿瘤生长。这些结果表明，林恩酪氨酸激酶是一个致癌基因，可以作为一个新的靶点，为宫颈癌的研究和治疗。
Cervical cancer is one of the most common malignant tumor in women. The mechanisms of cervical cancer are intricate and have not been fully understood. Therefore, we employed iTRAQ to obtain novel proteins profile which participates in the tumor oncogenesis of cervical cancer. 3300 proteins were identified aberrantly expressed in cervical cancer, and western bolt was performed to validate the results of iTRAQ. Then, we selected LYN for further study. Immunohistochemistry identified that LYN expression was significantly increased in cervical cancer tissues than that in cancer adjacent normal cervical tissues and normal cervical tissues. The increased LYN expression was significantly correlated with cancer differentiation and FIGO stage. Silencing LYN inhibited cell proliferation, migration and invasion, conversely, overexpression LYN promoted cell proliferation, migration and invasion. In terms of mechanism, LYN could also promote cervical cancer cells metastasis through activating IL-6/STAT3 pathway. In vivo study, overexpression LYN promoted tumor growth, meanwhile knockdown LYN inhibited tumor growth. These results indicate that LYN tyrosine kinase is an oncogenic gene and can serve as a novel target for cervical cancer research and therapy.