Murine lung eosinophil activation and chemokine production in allergic airway inflammation.
Murine lung eosinophil activation and chemokine production in allergic airway inflammation.
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DOI：
10.1038/cmi.2010.31
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发表时间：
2010-09
影响因子：
24.1
通讯作者：
中科院分区：
文献类型：
--
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嗜酸性粒细胞在哮喘和肺部感染中起重要作用。小鼠模型被广泛用于评估嗜酸性粒细胞在这些疾病中的功能意义和机制基础。然而，关于组织中的嗜酸性粒细胞处于内稳态的情况知之甚少。此外，几乎没有关于过敏性呼吸道炎症期间嗜酸性粒细胞趋化因子产生的数据。本研究比较了动态型和动态型嗜酸性粒细胞的性质和功能。正常组织中的嗜酸性粒细胞表达共刺激分子B7-1、B7-2和ICAM-1呈递抗原，但很少表达主要组织相容性复合体(MHC)II类分子，对T细胞增殖的刺激作用较差。这些嗜酸性粒细胞高水平表达趋化因子C10、巨噬细胞炎性蛋白-1α、巨噬细胞炎性蛋白-1γ、巨噬细胞趋化蛋白-2、嗜酸性粒细胞趋化因子和单核细胞趋化蛋白-5，并产生趋化因子蛋白。在体外培养中，嗜酸性粒细胞内的趋化因子迅速减少，伴随着表面标志的下调，这与零星的脱颗粒一致。过敏性气道炎小鼠肺嗜酸性粒细胞趋化因子mRNA表达增加，趋化因子蛋白产生增加，MHC-II和CD11c表达上调。他们也被发现是炎症肺中CCRl配体CCL6/C10和CCL9/MIP-1γ的主要产生者。C10和MIP-1γ的嗜酸性粒细胞产生与CD11b在变态反应性气道炎症时高肺树突状细胞的显著内流以及这些树突状细胞上CCR1R的高表达有关。这项研究提供了组织嗜酸性粒细胞的基线信息，记录了激活的嗜酸性粒细胞激活标志物和趋化因子的产生上调，并表明嗜酸性粒细胞是过敏性肺部炎症中产生趋化因子的关键细胞类型。
Eosinophils play important roles in asthma and lung infections. Murine models are widely used for assessing the functional significance and mechanistic basis for eosinophil involvements in these diseases. However, little is known about tissue eosinophils in homeostasis. In addition, little data on eosinophil chemokine production during allergic airway inflammation are available. In this study, the properties and functions of homeostatic and activated eosinophils were compared. Eosinophils from normal tissues expressed costimulation and adhesion molecules B7-1, B7-2 and ICAM-1 for Ag presentation but little major histocompatibility complex (MHC) class II, and were found to be poor stimulators of T-cell proliferation. However, these eosinophils expressed high levels of chemokine mRNA including C10, macrophage inflammatory protein (MIP)-1α, MIP-1γ, MIP-2, eotaxin and monocyte chemoattractant protein-5 (MCP-5), and produced chemokine proteins. Eosinophil intracellular chemokines decreased rapidly with concomitant surface marker downregulation upon in vitro culturing consistent with piecemeal degranulation. Lung eosinophils from mice with induced allergic airway inflammation exhibited increased chemokines mRNA expression and chemokines protein production and upregulated MHC class II and CD11c expression. They were also found to be the predominant producers of the CCR1 ligands CCL6/C10 and CCL9/MIP-1γ in inflamed lungs. Eosinophil production of C10 and MIP-1γ correlated with the marked influx of CD11bhigh lung dendritic cells during allergic airway inflammation and the high of CCR1 on these dendritic cells (DCs). The study provided baseline information on tissue eosinophils, documented the upregulation of activation markers and chemokine production in activated eosinophils, and indicated that eosinophils were a key chemokine-producing cell type in allergic lung inflammation.
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影响因子：
15.3
作者：
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