Modulation of neutrophil apoptosis by plasma and peritoneal fluid from patients with advanced endometriosis
Modulation of neutrophil apoptosis by plasma and peritoneal fluid from patients with advanced endometriosis
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DOI：
10.1093/humrep/17.3.595
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发表时间：
2002-03-01
影响因子：
6.1
通讯作者：
Lee, KS
中科院分区：
文献类型：
--
作者：
Kwak, JY;Park, SW;Lee, KS
背景：子宫内膜异位症中中性粒细胞促炎趋化细胞因子的产生增加表明免疫系统的变化在子宫内膜异位症的病理生理学中发挥重要作用。研究了晚期子宫内膜异位症患者血浆和腹腔液对中性粒细胞凋亡的影响。方法：吉姆萨染色通过形态学变化评价中性粒细胞凋亡变化。通过DNA电泳分析证实细胞凋亡。结果：与健康对照的血浆（n = 20）和腹膜液（n = 5）相比，将子宫内膜异位症患者的10%血浆（n = 20）和腹膜液（n = 10）添加到健康受试者的中性粒细胞体外培养物中，凋亡细胞的百分比从65.3 +/- 6.6降低到27.2 +/- 4.6% (P < 0.001) 和 45.3 ± 4.8 至 10.5 ± 4.3% (P < 0.001)。中和白细胞介素8抗体可以消除腹腔液诱导的中性粒细胞凋亡的延迟，但不能消除子宫内膜异位症患者血浆中的延迟。结论：这些研究结果表明，白细胞介素-8是子宫内膜异位症患者腹腔液中的中性粒细胞生存因子之一，并且子宫内膜异位症患者血浆中也存在一种未鉴定的生存因子。
BACKGROUND: The increased production of pro-inflammatory chemoattractant cytokines for neutrophils in endometriosis suggests that changes in the immune system play an important role in the pathophysiology of endometriosis. The effects of plasma and peritoneal fluid from patients with advanced endometriosis on the apoptosis of neutrophils were investigated. METHODS: Apoptotic changes of neutrophils were evaluated by morphological changes using Giemsa staining. Apoptosis was confirmed by DNA electrophoretic analysis. RESULTS: Compared with the plasma (n = 20) and peritoneal fluid (n = 5) of healthy controls, the addition of 10% plasma (n = 20) and peritoneal fluid (n = 10) from patients with endometriosis to an in-vitro culture of neutrophils from healthy subjects reduced the percentage of apoptotic cells from 65.3 +/- 6.6 to 27.2 +/- 4.6% (P < 0.001) and from 45.3 ± 4.8 to 10.5 ± 4.3% (P < 0.001) respectively. Neutralizing interleukin-8 antibody abrogated the delay of neutrophil apoptosis induced by peritoneal fluid, but not in the plasma of endometriosis patients. CONCLUSIONS: These findings show that interleukin-8 is one of the neutrophil survival factors in the peritoneal fluid of endometriosis patients and that an unidentified survival factor is also present in the plasma of patients with endometriosis.