In vitro activation of human herpesviruses 6 and 7 from latency
In vitro activation of human herpesviruses 6 and 7 from latency
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DOI：
10.1073/pnas.93.18.9788
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发表时间：
1996-09-03
影响因子：
11.1
通讯作者：
Frenkel, N
中科院分区：
文献类型：
--
作者：
Katsafanas, GC;Schirmer, EC;Frenkel, N
人类疱疹病毒6型和7型（HHV-6和HHV-7）是一种流行的嗜淋巴细胞病毒，在婴儿期或幼儿期感染超过80%的儿童。感染范围从无症状到严重疾病。HHV-6 B可引起急性皮疹，在急性皮疹期可从外周血单个核细胞中回收病毒，但宿主终生潜伏感染。在接受肾、肝或骨髓移植的免疫功能低下的患者中，潜伏的HHV-6 B被重新激活，有时会导致严重或致命的疾病。在这里，我们描述了一个体外系统的建立，从潜伏期的HHV-6 B和HHV-7的再激活。HHV-7通过T细胞活化从潜伏感染的外周血单核细胞中再活化。HHV-6 B在类似条件下不能被重新激活;然而，潜伏的HHV-6 B可以在用HHV-7感染细胞后恢复。一旦重新激活，HHV-6 B基因组变得突出，HHV-7消失。我们的结论是，HHV-7可以提供一个transacting功能（S）介导HHV-6从潜伏期重新激活。了解激活过程对于开发控制潜伏病毒激活的治疗方法至关重要，以避免这些有时危及移植受者生命的感染。
Human herpesviruses 6 and 7 (HHV-6 and HHV-7) are prevalent lymphotropic viruses that infect more than 80% of children at infancy or during early childhood. Infection ranges from asymptomatic to severe disease. HHV-6B causes exanthem subitum, The virus can be recovered from peripheral blood mononuclear cells during the acute phase of exanthem subitum, but the host remains latently infected throughout life. In immunocompromised patients undergoing kidney, liver, or bone marrow transplantation latent HHV-6B is reactivated, at times causing severe or fatal disease. Here, we describe the establishment of an in vitro system for reactivation of HHV-6B and HHV-7 from latency. HHV-7 is reactivated from latently infected peripheral blood mononuclear cells by T-cell activation. HHV-6B could not be reactivated under similar conditions; however, the latent HHV-6B could be recovered after the cells were infected with HHV-7. Once reactivated, the HHV-6B genomes became prominent and the HHV-7 disappeared. We conclude that HHV-7 can provide a transacting function(s) mediating HHV-6 reactivating from latency. Understanding the activation process is critical for the development of treatments to control the activation of latent viruses so as to avoid these sometimes life threatening infections in transplant recipients.