Manipulation and Overstretching of Genes on Solid Substrates
Manipulation and Overstretching of Genes on Solid Substrates
复制标题
固体基质上基因的操纵和过度拉伸
DOI：
10.1021/nl035147d
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发表时间：
2004
期刊：
影响因子：
10.8
通讯作者：
J. Rabe
中科院分区：
文献类型：
--
作者：
N. Severin;J. Barner;A. Kalachev;J. Rabe
“分子芯片”，即单个大分子自由排列的芯片，是制造纳米级分子器件（如DNA分子阵列芯片）的关键。我们报告了一种操作已经沉积的单个大分子的新方法，该方法允许在底物上自由定位单个聚电解质（如DNA），弯曲和拉伸它们，去除拉伸缺陷，并将双链DNA过度拉伸成两条平行的单链。
A “molecular chip”, i.e., a chip on which single macromolecules are freely arranged, is a key for the fabrication of nanoscopic molecular devices, e.g., DNA molecular array chips. We report on a new method for the manipulation of already deposited single macromolecules, which allows to freely position single polyelectrolytes such as DNA on a substrate, to bend and stretch them, to remove stretching defects, and to overstretch double stranded DNA into two parallel single strands.