Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic Nephropathy.
Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic Nephropathy.
复制标题
尿蛋白糖模式作为糖尿病肾病新的潜在诊断指标
DOI：
10.1155/2017/5728087
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发表时间：
2017
影响因子：
4.3
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Zhu H;Liu M;Yu H;Liu X;Zhong Y;Shu J;Fu X;Cai G;Chen X;Geng W;Yang X;Wu M;Li Z;Zhang D
糖尿病肾病是慢性肾病和终末期肾病的主要原因。然而，对糖尿病肾病发生发展过程中尿中糖蛋白的变化知之甚少。目前，我们使用凝集素微阵列询问尿液标本中的糖蛋白。结果表明，随着糖尿病肾病的发展，SNA识别的Siaα2- 6 Gal/GalNAc表达水平呈明显升高趋势，而SNA印迹法检测尿中90 kDa、70 kDa和40 kDa的糖蛋白可能参与了这一变化。此外，STL识别的（GlcNAc）2-4的糖蛋白在糖尿病肾病和非糖尿病肾病之间存在差异。另外48例尿标本制备的尿蛋白芯片结果也表明（GlcNAc）2-4是鉴别糖尿病肾病与非糖尿病肾病的潜在指标。STL印迹分析表明，50 kDa的糖蛋白与此变化相关。总之，我们的数据提供了关键的信息，以监测糖尿病肾病的发展和区分糖尿病肾病和非糖尿病肾病的基础上，精确改变尿蛋白中的糖蛋白，但在这方面需要进一步的研究。
Diabetic nephropathy is a major cause of chronic kidney disease and end-stage kidney disease. However, so little is known about alterations of the glycopatterns in urine with the development of diabetic nephropathy. Presently, we interrogated glycopatterns in urine specimens using a lectin microarray. The results showed that expression levels of Siaα2-6Gal/GalNAc recognized by SNA exhibited significantly increased tendency with the development of diabetic nephropathy; moreover, SNA blotting indicated glycoproteins (90 kDa, 70 kDa, and 40 kDa) in urine may contribute to this alteration. Furthermore, the glycopatterns of (GlcNAc)2–4 recognized by STL exhibited difference between diabetic and nondiabetic nephropathy. The results of urinary protein microarray fabricated by another 48 urine specimens also indicated (GlcNAc)2–4 is a potential indictor to differentiate the patients with diabetic nephropathy from nondiabetic nephropathy. Furtherly, STL blotting showed that the 50 kDa glycoproteins were correlated with this alteration. In conclusion, our data provide pivotal information to monitor the development of diabetic nephropathy and distinguish between diabetic nephropathy and nondiabetic renal disease based on precise alterations of glycopatterns in urinary proteins, but further studies are needed in this regard.