Study of male sterility in Taiwania cryptomerioides Hayata (Taxodiaceae)
Study of male sterility in Taiwania cryptomerioides Hayata (Taxodiaceae)
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DOI：
10.1007/s00709-006-0166-2
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发表时间：
2006-08
期刊：
影响因子：
2.9
通讯作者：
S.-H. Chen;Nien-June Chung;Y.-N. Wang;Clifford Y. L. Lee;Y.-L. Lee;Po-Fang Tsai
中科院分区：
文献类型：
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作者：
S.-H. Chen;Nien-June Chung;Y.-N. Wang;Clifford Y. L. Lee;Y.-L. Lee;Po-Fang Tsai
本文对台湾特有的针叶树种台湾杉科（Taxodiaceae）进行了连续三年的雄性不育研究。借助荧光和电子显微镜观察，使用从台湾南投国立台湾大学溪头实验林和汝秀坑无性系园采集的样本，比较了可育和不育小孢子囊的个体发育过程。雄性球果的发育发生在8月至3月底。小孢子发生开始于原孢子囊的形成，结束于开裂小孢子囊内的 2 细胞花粉粒的成熟。减数分裂前，可育和不育小孢子囊的超微结构无显着差异。具有各种四分体形式的异步花粉发育可能发生在可育或不育球果的同一小孢子囊中。然而，在可育的二分体和四分体中可以观察到胼胝质壁，但在不育的二分体中观察不到。胼胝质壁溶解后，可育的小孢子被释放到子房中，而一些不育的小孢子仍然保留为四分体或二分体，在近端面的外壁相互缠绕。结果，无菌小孢子中没有发育良好的片状内壁，也没有颗粒状外壁或内壁。最终，不育小孢子的细胞内结构在开花前急剧崩溃。目前的研究表明，花粉发育的失败可能归因于胼胝质壁的缺失。这种结构对于T的雄性不育的重要性。讨论了柳杉病。
A study of male sterility over a period of three consecutive years on a conifer species endemic to Taiwan,Taiwania cryptomerioidesHayata (Taxodiaceae), was done for this article. With the aids of fluorescence and electron microscopic observations, the ontogenic processes in the fertile and sterile microsporangia are compared, using samples collected from Chitou Experimental Forest and Yeou-Shoei-Keng Clonal Orchard of the National Taiwan University, Nantou, Taiwan. The development of male strobili occurred from August to the end of March. Microsporogenesis starts with the formation of the archesporium and ends with the maturation of 2-celled pollen grains within the dehiscing microsporangium. Before meiosis, there was no significant difference in ultrastructure between the fertile and sterile microsporangia. Asynchronous pollen development with various tetrad forms may occur in the same microsporangium of either fertile or sterile strobili. However, a callose wall was observable in the fertile dyad and tetrad, but not in the sterile one. After dissolution of the callose wall, the fertile microspores were released into the locule, while some sterile microspores still retained as tetrads or dyads with intertwining of exine walls in the proximal faces. As a result, there was no well developed lamellated endexine and no granulate ectexine or intine in the sterile microspores. Eventually, the intracellular structures in sterile microspores were dramatically collapsed before anthesis. The present study shows that the abortion in pollen development is possibly attributed to the absence of the callose wall. The importance of this structure to the male sterility ofT. cryptomerioidesis discussed.