Progesterone administration in vivo stimulates release of luteinizing hormone-releasing hormone in vitro.
Progesterone administration in vivo stimulates release of luteinizing hormone-releasing hormone in vitro.
复制标题
体内给予黄体酮会刺激体外黄体生成素释放激素的释放。
作者：
V. Ramirez;D. Dluzen;D. Lin
用灌流系统测定了青春期前雌性大鼠下丘脑内侧基底核-下丘脑前部-视前区组织中促黄体生成素释放激素（LHRH）的释放。还测量了这些组织碎片中LHRH的浓度和处死大鼠时获得的血清中促黄体生成素的浓度。四组未成年大鼠进行了研究：完整的，卵巢切除，卵巢切除和植入雌二醇胶囊，卵巢切除大鼠与雌二醇和注射孕酮。从卵巢切除动物的组织中释放的LHRH显着低于完整的女性，并没有修改时，卵巢切除大鼠接受雌二醇。然而，在注射孕酮6小时后处死的卵巢切除大鼠中，雌二醇引发的组织中LHRH的释放增加了4 - 5倍。组织中的LHRH和血清中的促黄体生成素的浓度在组间和动物被处死的一天中的时间不同。黄体生成素、性腺类固醇和光周期之间的相互作用似乎为触发黄体生成素完全和正常释放的神经过程设置了适当的条件。
The release of luteinizing hormone-releasing hormone (LHRH) from tissue from the mediobasal hypothalamic-anterior hypothalamic-preoptic area of prepuberal female rats was measured in a perfusion system. Measurements were also made of the concentrations of LHRH in these tissue fragments and of luteinizing hormone in serum obtained when the rats were killed. Four groups of immature rats were studied: intact, ovariectomized, ovariectomized and implanted with estradiol-containing capsules, and ovariectomized rats primed with estradiol and injected with progesterone. The release of LHRH from the tissue of ovariectomized animals was significantly less than that of intact females and was not modified when the ovariectomized rats received estradiol. However, there was a four- to fivefold increase in LHRH release from tissue of ovariectomized rats primed with estradiol when they were killed 6 hours after they received an injection of progesterone. The concentrations of LHRH in tissue and of luteinizing hormone in serum varied among groups and with the time of day that the animals were killed. The interactions among luteinizing hormone, gonadal steroids, and the photoperiod seem to set the appropriate conditions for neural processes triggering a complete and normal release of luteinizing hormone.