THE PLASMA LIPOPROTEINS: HISTORICAL DEVELOPMENTS AND NOMENCLATURE *
THE PLASMA LIPOPROTEINS: HISTORICAL DEVELOPMENTS AND NOMENCLATURE *
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血浆脂蛋白：历史发展和命名*
DOI：
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发表时间：
1980
影响因子：
5.2
通讯作者：
F. Lindgren
中科院分区：
文献类型：
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作者：
F. Lindgren
我很高兴也很荣幸今天能在这里就血清脂蛋白的历史发展和命名提供一些想法、回忆和结论。正如通常的情况一样，新的方法和技术改进先行，并且对于应用和突破来说是必要的。第一个里程碑是在 1924 年，当时 Svedberg 和 Rinde 发明了超速离心机，用于研究金胶体的粒径。1 随后，在 1927 年，Svedberg 和 Lysholm 开发了一种用于蛋白质分析的更高速的油轮机分析超速离心机，它允许通过光学系统检测迁移边界。图 1 显示了第一台低速直接驱动电气分析超速离心机。此后，进行了许多改进和完善2 大约在同一时间，Macheboef 首次对马血清脂蛋白进行了化学分离。与后来的几年一样，该定义是可操作的，即“酸共沉淀”或使用半饱和硫酸铵的 CA。 1944 年晚些时候，在电泳技术被开发出来之后，他报告说它是一种 α 球蛋白，这表明第一个分离的脂蛋白是高密度脂蛋白 (HDL)。
It is my pleasure and honor to be here today to offer a few thoughts, recollections, and conclusions on the historical developments and nomenclature of the serum lipoproteins. As is usually the case, new methodology and technical improvements preceded and were necessary for applications and breakthroughs. The first landmark was in 1924, when Svedberg and Rinde invented the ultracentrifuge with application to the study of particle size of gold colloids.1 Later, in 1927, Svedberg and Lysholm developed a higher speed oil turbine analytic ultracentrifuge for protein analysis, which allowed detection of migrating boundaries by an optical system. FIGURE 1 shows one of the first low-speed direct-drive electrical analytic ultracentrifuges. Thereafter, there were many improvements and refinements2 About the same time, Macheboef performed the first chemical separation of a horse serum lipoprotein. The definition was, as in later years, operational, i.e., “coenapse precipitated by acid” or CA using half-saturated ammonium sulfate. Much later in 1944, after electrophoresis had been developed, he reported it to be an a, globulin, which now would indicate this first isolated lipoprotein to be a high-density lipoprotein (HDL) .