Electrospun gelatin/polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and chondrocytes for cartilage engineering.
Electrospun gelatin/polycaprolactone nanofibrous membranes combined with a coculture of bone marrow stromal cells and chondrocytes for cartilage engineering.
复制标题
电纺明胶/聚己内酯纳米纤维膜与骨髓基质细胞和软骨细胞共培养相结合，用于软骨工程。
DOI：
10.2147/ijn.s79461
复制
发表时间：
2015
影响因子：
8
通讯作者：
Zheng J
中科院分区：
文献类型：
--
作者：
He X;Feng B;Huang C;Wang H;Ge Y;Hu R;Yin M;Xu Z;Wang W;Fu W;Zheng J
静电纺丝最近受到了相当大的关注，作为软骨工程支架制造的新方法显示出巨大的潜力。本研究的目的是使用软骨细胞与电纺明胶/聚己内酯（GT/PCL）膜相结合的共培养策略，而不是纯软骨细胞，来评估软骨组织的形成。我们在体外三明治模型中制备了 GT/PCL 膜、接种骨髓基质细胞 (BMSC)/软骨细胞共培养物（75% BMSC 和 25% 软骨细胞），然后将构建体皮下植入裸鼠体内 12 周。植入后进行了肉眼观察、组织学和免疫组织学评估、糖胺聚糖分析、杨氏模量测量和免疫荧光染色。我们发现共培养组形成了成熟的软骨样组织，与软骨细胞组无统计学差异，并且标记的BMSCs可以在软骨细胞的软骨形成微环境下分化为软骨细胞样细胞。整个策略表明 GT/PCL 膜也是基于干细胞的软骨工程的合适支架，并可能为软骨修复提供潜在的临床可行方法。
Electrospinning has recently received considerable attention, showing notable potential as a novel method of scaffold fabrication for cartilage engineering. The aim of this study was to use a coculture strategy of chondrocytes combined with electrospun gelatin/polycaprolactone (GT/PCL) membranes, instead of pure chondrocytes, to evaluate the formation of cartilaginous tissue. We prepared the GT/PCL membranes, seeded bone marrow stromal cell (BMSC)/chondrocyte cocultures (75% BMSCs and 25% chondrocytes) in a sandwich model in vitro, and then implanted the constructs subcutaneously into nude mice for 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan analyses, Young’s modulus measurement, and immunofluorescence staining were performed postimplantation. We found that the coculture group formed mature cartilage-like tissue, with no statistically significant difference from the chondrocyte group, and labeled BMSCs could differentiate into chondrocyte-like cells under the chondrogenic niche of chondrocytes. This entire strategy indicates that GT/PCL membranes are also a suitable scaffold for stem cell-based cartilage engineering and may provide a potentially clinically feasible approach for cartilage repairs.