On the specific toxicity of 5-thio-D-glucose to hypoxic cells.
On the specific toxicity of 5-thio-D-glucose to hypoxic cells.
复制标题
5-硫代-D-葡萄糖对缺氧细胞的特异性毒性。
作者：
R. Schulz;P. Bongiorni
已经从氧张力、浓度和温度方面研究了5 - 硫代 - D - 葡萄糖（5TG）对培养的哺乳动物细胞的毒性。在37℃以及正常生长培养基中药物浓度为5 mM时，在5 ppm O₂中暴露4小时的存活率为10⁻³；在200 ppm O₂中暴露24小时，存活率则增至0.5。存活率与氧张力之间的关系是非线性的，最大变化出现在50至100 ppm之间。该药物对有氧细胞基本无毒。药物毒性随浓度增加而增大，直至约5 mM时达到一个平台期。升高温度的作用是减少达到特定存活水平所需的时间，但对于氧张力高于100 ppm的情况，高达42℃的温度对药物毒性只有轻微影响。研究了D - 葡萄糖对5TG毒性的影响，并确定了一种反比关系。当D - 葡萄糖浓度大于20 mM时，无论氧张力或5TG浓度如何，5TG的毒性都被消除。
The toxicity of 5-thio-D-glucose (5TG) to mammalian cells in culture has been studied with respect to oxygen tension, concentration, and temperature. At 37 degrees C and at 5 mM concentration of the drug in normal growth medium, survival is 10(-3) for 4-hr exposure to 5 ppm O2; this increases to 0.5 for 24-hr exposure to 200 ppm O2. The relationship between survival and oxygen tension is nonlinear with the greatest change occurring between 50 and 100 ppm. The drug is essentially nontoxic to aerated cells. Drug toxicity increases with concentration up to about 5 mM at which point a plateau is reached. The effect of elevated temperature is to reduce the time required to obtain a specific level of survival, but temperatures as high as 42 degrees C had only a slight effect on drug toxicity for oxygen tensions higher than 100 ppm. The effect of D-glucose on the toxicity of 5TG was studied, and an inverse relationship was established. At D-glucose concentrations greater than 20 mM the toxicity of 5TG was nullified regardless of oxygen tension or 5TG concentration.