Influence of low dietary histamine on the seizure development of chemical kindling induced by pentylenetetrazol in rats
Influence of low dietary histamine on the seizure development of chemical kindling induced by pentylenetetrazol in rats
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DOI：
10.1111/j.1745-7254.2005.00097.x
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发表时间：
2005-04
影响因子：
8.2
通讯作者：
Chun-lei Jin;E. Sakurai;Y. Kiso;Jian‐hong Luo;K. Yanai;Zhong Chen
中科院分区：
文献类型：
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作者：
Chun-lei Jin;E. Sakurai;Y. Kiso;Jian‐hong Luo;K. Yanai;Zhong Chen
摘要：探讨低组胺饮食对戊四唑（PTZ）诱发大鼠癫痫发作的影响。方法：低组胺日粮（LH，含组胺0.145 μmol/g）饲喂14 d后，每48 h腹腔重复注射亚惊厥剂量PTZ (35 mg/kg) 1次，记录30 min的癫痫发作活动。采用高效液相色谱系统和荧光分光光度仪对脑样品中的组胺进行了分析。结果：LH饮食增加了PTZ第一次试验的癫痫发作反应（癫痫易感性），并促进了PTZ随后的点燃过程（癫痫发作发展）。lh治疗大鼠的皮层、海马和下丘脑组胺水平显著降低，且与癫痫发作行为相关（r分别为0.875、0.651和0.796）。此外，慢性点燃性癫痫发作导致lh喂养组皮层和下丘脑组胺含量显著增加。结论：日常食物中的组胺可影响大鼠脑组胺能功能，并在调节癫痫易感性中发挥重要作用。
AbstractAim:To determine the role of dietary low histamine on the seizure development of pentylenetetrazol (PTZ)-induced kindling in rats.Methods:After 14 d of feeding on a low histamine diet (LH, containing 0.145 μmol/g of histamine), the rats were chemically kindled by repeated intraperitoneal injection of a subconvulsant dose of PTZ (35 mg/kg) once every 48 h, and seizure activity of kindling was recorded for 30 min. Histamine in brain samples was analyzed using a high performance liquid chromatography system with a fluorescence spectrofluorometer.Results:The LH diet induced an increase in seizure response (seizure susceptibility) to the first trial of PTZ, and resulted in facilitation of subsequent PTZ kindling process (seizure development). The histamine levels in the cortex, hippocampus, and hypothalamus of LH-treated rats decreased significantly and these changes correlated well with seizure behavior (r= 0.875, 0.651, and 0.796, respectively). In addition, chronic kindled seizures resulted in a significant increase of the histamine content in the cortex and hypothalamus in the LH-fed groups.Conclusion:These findings indicate that the histamine in daily food could influence the brain histaminergic function, and play an important role in regulating seizure susceptibility.