Detection of neuropeptides in vivo and open questions for current and upcoming fluorescent sensors for neuropeptides
Detection of neuropeptides in vivo and open questions for current and upcoming fluorescent sensors for neuropeptides
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DOI：
10.1016/j.peptides.2020.170456
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发表时间：
2021-02-01
期刊：
影响因子：
3
通讯作者：
Onaka, Tatsushi
中科院分区：
文献类型：
--
作者：
Inutsuka, Ayumu;Ino, Daisuke;Onaka, Tatsushi
在应激反应期间，大脑中以时空协调的方式分泌各种神经肽。为了准确了解应激反应中肽的功能，重要的是研究它们释放的时间和地点、它们如何扩散以及它们如何在大脑中分解。在过去的二十年中，基因编码的荧光钙指示剂极大地提高了我们对特定神经元活动在调节应激期间行为变化和生理反应中的功能的认识。此外，神经肽G蛋白偶联受体（GPCR）的各种结构信息也已被揭示。最近，已经开发出基因编码的荧光传感器，通过利用配体结合诱导的构象变化来检测神经递质。在这篇综述中，我们总结了最近和即将到来的神经肽检测技术的进展，然后提出了几个悬而未决的问题，这些问题将通过应用最近或即将到来的神经肽体内检测技术进展来解决。
During a stress response, various neuropeptides are secreted in a spatiotemporally coordinated way in the brain. For a precise understanding of peptide functions in a stress response, it is important to investigate when and where they are released, how they diffuse, and how they are broken down in the brain. In the past two decades, genetically encoded fluorescent calcium indicators have greatly advanced our knowledge of the functions of specific neuronal activity in regulation of behavioral changes and physiological responses during stress. In addition, various kinds of structural information on G-protein-coupled receptors (GPCRs) for neuropeptides have been revealed. Recently, genetically encoded fluorescent sensors have been developed for detection of neurotransmitters by making use of conformational changes induced by ligand binding. In this review, we summarize the recent and upcoming advances of techniques for detection of neuropeptides and then present several open questions that will be solved by application of recent or upcoming technical advances in detection of neuropeptides in vivo.