Mutation of the rice narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport
Mutation of the rice narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport
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编码新型蛋白质的水稻窄叶1基因突变影响叶脉图案和极性生长素运输
DOI：
10.1104/pp.108.118778
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发表时间：
2008-08-01
期刊：
影响因子：
7.4
通讯作者：
Li, Chuanyou
中科院分区：
文献类型：
--
作者：
Qi, Jing;Qian, Qian;Li, Chuanyou
植物叶片的大小和形状是重要的农艺性状。为了了解植物叶片形状的分子机制，我们描述了一个经典的水稻（Oryza sativa）矮秆突变体命名为窄叶1（nal 1），它表现出窄叶的特征表型。与叶片宽度的减小相一致，nal1叶片的纵脉数量减少。解剖学研究表明，nal1的秆也表现出缺陷的维管系统，其中维管束的数量和分布格局被改变。图位克隆和遗传互补分析表明，Nal 1编码一种植物特异性蛋白，其生化功能未知。我们提供的证据表明，Nal 1是丰富的表达在维管组织和该基因的突变导致显着降低极性生长素运输能力。这些结果表明，Nal 1影响生长素的极性运输以及水稻植株的维管格局，并在控制侧叶生长中起重要作用。
The size and shape of the plant leaf is an important agronomic trait. To understand the molecular mechanism governing plant leaf shape, we characterized a classic rice ( Oryza sativa) dwarf mutant named narrow leaf1 (nal1), which exhibits a characteristic phenotype of narrow leaves. In accordance with reduced leaf blade width, leaves of nal1 contain a decreased number of longitudinal veins. Anatomical investigations revealed that the culms of nal1 also show a defective vascular system, in which the number and distribution pattern of vascular bundles are altered. Map-based cloning and genetic complementation analyses demonstrated that Nal1 encodes a plant-specific protein with unknown biochemical function. We provide evidence showing that Nal1 is richly expressed in vascular tissues and that mutation of this gene leads to significantly reduced polar auxin transport capacity. These results indicate that Nal1 affects polar auxin transport as well as the vascular patterns of rice plants and plays an important role in the control of lateral leaf growth.