Phosphohistone H3 and Ki-67 Labeling Indices in Cytologic Specimens from Well-Differentiated Neuroendocrine Tumors of the Gastrointestinal Tract and Pancreas: A Comparative Analysis Using Automated Image Cytometry
Phosphohistone H3 and Ki-67 Labeling Indices in Cytologic Specimens from Well-Differentiated Neuroendocrine Tumors of the Gastrointestinal Tract and Pancreas: A Comparative Analysis Using Automated Image Cytometry
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DOI：
10.1159/000351475
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发表时间：
2013-01-01
期刊：
影响因子：
1.8
通讯作者：
Reid, Michelle D.
中科院分区：
文献类型：
--
作者：
Fung, Adele D.;Cohen, Cynthia;Reid, Michelle D.
背景资料：Ki-67增殖指数最近被纳入胃肠道（GIT）和胰腺神经内分泌肿瘤（NEN）的分级。这些肿瘤现在被分为高分化神经内分泌肿瘤（WDN，1级和2级）和低分化神经内分泌癌（3级）。虽然Ki-67是NEN中已确定的增殖标记物，但磷酸组蛋白H3（PHH 3），一种较新的有丝分裂活性标记物，不是。研究方法：我们使用自动细胞成像系统（ACIS（R））测定了GIT和胰腺WDNV细胞学样本的Ki-67和PHH 3指数。结果：细胞学标本上ACIS产生的Ki-67和PHH 3指数之间有很强的相关性。然而，在某些情况下，这两种染色剂在同一肿瘤中引起相互冲突的等级。结论：两种抗体均对细胞周期不同阶段的细胞进行染色，这可能导致分级不一致，从而影响患者管理和诊断。Ki-67染色比PHH 3更强，使得在ACIS上更容易识别“热点”。Ki-67比PHH 3更适合用于染色NEN，特别是在具有边界等级的肿瘤中。由于PHH 3产生较低的有丝分裂指数，因此在其表达被进一步表征之前，不应将其用作NEN中的增殖标志物。(C)2013 S. Karger AG，巴塞尔
Background: Ki-67 proliferation index was recently incorporated in the grading of neuroendocrine neoplasms (NENs) of the gastrointestinal tract (GIT) and pancreas. These are now divided into well-differentiated neuroendocrine tumors (WDNETs, grades 1 and 2) and poorly differentiated neuroendocrine carcinomas (grade 3). While Ki-67 is an established proliferation marker in NENs, phosphohistone H3 (PHH3), a newer marker of mitotic activity, is not. Methods: We determined Ki-67 and PHH3 indices on cytologic samples from WDNETs of the GIT and pancreas using an automated cellular imaging system (ACIS (R)). Results:There was a strong correlation between Ki-67 and PHH3 indices generated by ACIS on cytologic samples. However, in some cases the two stains caused conflicting grades within the same tumor. Conclusion: Both antibodies stain cells in different phases of the cell cycle which may cause discordant grades, thus affecting patient management and prognostication. Ki-67 staining is stronger than PHH3, making 'hot spots' easier to identify on ACIS. Ki-67 is more ideal than PHH3 for staining NENs, especially in tumors with borderline grades. Because PHH3 generates lower mitotic indices it should not be used as a proliferation marker in NENs until its expression has been further characterized. (C) 2013 S. Karger AG, Basel