Differential regulation of MAPK (JNK 3) gene expression in human head and neck squamous cell carcinomas
Differential regulation of MAPK (JNK 3) gene expression in human head and neck squamous cell carcinomas
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DOI：
10.1159/000079087
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发表时间：
2004-01-01
期刊：
影响因子：
0.3
通讯作者：
Lippert, BM
中科院分区：
文献类型：
--
作者：
Görögh, T;Berwig, J;Lippert, BM
背景资料：JNK 3基因编码一种属于丝裂原活化蛋白（MAP）激酶的蛋白质，MAP激酶是一组参与信号传导途径的分子。材料和方法：从头颈部鳞状细胞癌（HNSCC）细胞和正常上呼吸消化道粘膜角质形成细胞中提取信使RNA进行转录。对所得cDNA群体进行任意引物的mRNA指纹。电泳后，用放射自显影法检测带型。结果：在HNSCC细胞中检测到一个107 bp的mRNA片段，与良性表型相比，HNSCC细胞表现出明显的抑制作用。克隆该片段后，数据库搜索显示与属于c-jun N-末端MAP-10激酶（JNK 3）的序列具有精确同源性。北方杂交证实，与邻近正常粘膜相比，HNSCC活检组织中该基因的表达明显减少。结论：结果表明JNK 3基因的表达受到强烈抑制，提示JNK 3与头颈癌的致癌过程有关。
Background: The JNK 3 gene encodes a protein which belongs to the mitogen-activated protein ( MAP) kinases, a group of molecules involved in signaling pathways. Material and Methods: Messenger RNA extracted from head and neck squamous cell carcinoma (HNSCC) cells and normal upper aerodigestive tract mucosa keratinocytes was reversely transcribed. The resulting cDNA populations were subjected to an arbitrarily primed mRNA fingerprint. After electrophoresis, the band pattern was detected by autoradiography. Results: A 107 bp mRNA fragment was detected in HNSCC cells showing considerable repression in comparison with the benign phenotype. After cloning of this fragment, a database search revealed an exact homology with sequences belonging to the c-jun N-terminal MAP-10 kinase (JNK 3). Northern hybridization confirmed the distinctly reduced expression of this gene in HNSCC biopsies in contrast to adjacent normal mucosa. Conclusion: The results show evidence that the expression of the JNK 3 gene is strongly repressed, suggesting that JNK 3 is implicated in carcinogenic processes in head and neck cancer.