Stem cells isolated from human dental follicles have osteogenic potential
Stem cells isolated from human dental follicles have osteogenic potential
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DOI：
10.1016/j.tripleo.2010.08.004
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发表时间：
2011-06-01
期刊：
影响因子：
--
通讯作者：
Satomura, Kazuhito
中科院分区：
文献类型：
--
作者：
Honda, Masaki J.;Imaizumi, Mari;Satomura, Kazuhito
客观的。从人类牙囊中分离出的干细胞作为骨组织工程的潜在细胞来源进行了检查，以纠正严重的骨缺陷。研究设计。收集受影响的第三磨牙，并在生长培养基中培养单细胞来源的细胞群。对单细胞来源的细胞系的细胞形状、基因表达模式、体外分化能力和体内成骨潜力进行了检查。结果。鉴定出三种不同的细胞群，具有不同的形态、基因表达模式和分化能力。与未进行细胞移植的对照动物相比，当移植到免疫缺陷大鼠颅骨中通过手术产生的临界尺寸缺损时，所有 3 个细胞群都促进了骨形成，尽管其中一个细胞群在体外显示出较弱的成骨分化能力。结论。人类牙囊在培养物中可以衍生出至少 3 种独特的细胞群，所有这些细胞群都促进体内骨形成。 （口腔外科、口腔医学、口腔病理、口腔放射学 Endod 2011；111：700-708）
Objective. Stem cells isolated from human dental follicles as a potential cell source for bone-tissue engineering were examined for correcting a critical bone defect.Study design. Impacted third molars were collected and single cell-derived cell populations were cultivated in growth medium. Single cell-derived cell lines were examined in terms of cell shape, gene expression patterns, differentiation capacity in vitro, and osteogenic potential in vivo.Results. Three distinct cell populations were identified with different morphologies, patterns of gene expression, and differentiation capacity. All 3 cell populations promoted bone formation when transplanted into surgically created critical-size defects in imunodeficient rat calvaria, compared with control animals without cell transplantation, although one of these populations showed a weak capacity for osteogenetic differentiation in vitro.Conclusions. Human dental follicle can derive at least 3 unique cell populations in culture, all of which promote bone formation in vivo. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 111: 700-708)